Two copies of bla NDM-1 gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia |
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Authors: | Branko Jovčić Zorica Lepšanović Jelena Begović Brankica Filipić Milan Kojić |
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Affiliation: | 1. Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, P.O. Box 23, 11010, Belgrade, Serbia 2. Faculty of Biology, University of Belgrade, Studentski trg 16, 11000, Belgrade, Serbia 3. Military Medical Academy, Crnotravska 17, 11000, Belgrade, Serbia 4. Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000, Belgrade, Serbia
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Abstract: | New Delhi metallo-β-lactamase producing Pseudomonas aeruginosa isolates are of special interest since P. aeruginosa is a major cause of nosocomial infections, the treatment of which could now be jeopardized, especially in developing countries. Six additional NDM-1 positive P. aeruginosa clinical isolates belonging to two different genotypes were shown to be plasmid-free. PFGE-hybridization experiments revealed the chromosomal location of the bla NDM-1 gene. Restriction analysis and hybridization revealed that two copies of the bla NDM-1 gene are present in the genomes of all tested isolates, as in previously characterized P. aeruginosa MMA83. Moreover, it was shown that increasing imipenem concentration did not have the effect on copy number of the bla NDM-1 gene in the genome of P. aeruginosa MMA83. |
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