Methylation of the alpha-fetoprotein gene in isogenic rat hepatoma and liver cell lines |
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| Authors: | K Sakuma J R Cook C L Smith J F Chiu |
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| Affiliation: | 1. Department of Pharmacology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand;2. Veterinary Clinical Stem Cell and Bioengineering Research Unit, Veterinary Stem Cell and Bioengineering Innovation Center (VSCBIC), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand;3. Department of Anatomy, Center of Excellence for Regenerative Dentistry, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand;1. Computational and Systems Medicine, Department of Surgery and Cancer, Imperial College London, London SW7 2AZ, UK;2. Syngenta, Jealott’s Hill, Bracknell, Berkshire, RG42 6EY, UK;1. Division of Diabetes, Endocrinology & Gastroenterology, Faculty of Biology, Medicine & Health, AV Hill Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK;2. Endocrinology Department, Manchester University NHS Foundation Trust, Grafton Street, Manchester M13 9WU, UK;3. Bioinformatics Group, Faculty of Biology, Medicine & Health, University of Manchester, Oxford Road, Manchester M13 9PT, UK;4. Wellcome Trust Sanger Institute, Hinxton CB10 1SA, UK;5. Wellcome Trust-Medical Research Council Stem Cell Institute, Anne McLaren Laboratory and Department of Surgery, University of Cambridge, Cambridge CB2 0SZ, UK;6. Division of Dentistry, Faculty of Biology, Medicine & Health, University of Manchester, Oxford Road, Manchester M13 9PT, UK |
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| Abstract: | We have found that DNA methylation is inversely correlated with alpha-fetoprotein (AFP) gene expression in a series of isogenic rat hepatoma cell lines. The 5' end of the gene is extensively demethylated in AFP-producing cells and is highly methylated in cell lines which do not produce AFP. Glucocorticoid affects markedly the synthesis of AFP in the hepatoma cells. However, methylation patterns of cell lines which were treated with dexamethasone were not different from those of control cells, indicating that glucocorticoid action on AFP gene expression does not alter DNA methylation in this region of the gene. |
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