Recent advances in the generation of human monoclonal antibody |
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Authors: | Makiko Yamashita Yoshinroi Katakura Sanetaka Shirahata |
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Institution: | (1) Graduate School of Systems Life Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku Fukuoka, 812-8581, Japan;(2) Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku Fukuoka, 812-8581, Japan |
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Abstract: | The use of monoclonal antibodies (mAbs) has now gained a niche as an epochal breakthrough in medicine. Engineered antibodies
(Abs) currently account for over 30% of biopharmaceuticals in clinical trials. Several methods to generate human mAbs have
evolved, such as (1) immortalization of antigen-specific human B cell hybridoma technology, (2) generation of chimeric and
humanized antibody (Ab) from mouse Ab by genetic engineering, (3) acquisition of antigen-specific human B cells by the phage
display method, and (4) development of transgenic mice for producing human mAbs. Besides these technologies, we have independently
developed a method to generate human mAbs by combining the method of in vitro immunization using peripheral blood mononuclear
cells and the phage display method. In this paper, we review the developments in these technologies for generating human mAbs. |
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Keywords: | Human monoclonal antibody In vitro immunization Antibody engineering Humanization Antibody therapy |
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