Monoclonal antibody ECCD-1 inhibits intercellular communication in teratocarcinoma PCC3 cells |
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Authors: | Yoshinobu Kanno Yasuto Sasaki Yoshiki Shiba Chikako Yoshida-Noro Masatoshi Takeichi |
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Affiliation: | 1. Department of Physiology, Hiroshima University, School of Dentistry, Hiroshima 734 Japan;2. Department of Biophysics, Faculty of Science, Kyoto University, Kyoto 606, Japan |
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Abstract: | The monoclonal antibody ECCD-1 recognizing a certain class of cell surface proteins inhibits the Ca2+-dependent cell-to-cell adhesion in teratocarcinoma stem cells. In this paper, we studied the effect of ECCD-1 on cell-to-cell communication in PCC3 cells by measuring the transfer of lucifer yellow between cells. To this aim, PCC3 cells were cultured in the presence of ECCD-1 for various periods, and then the fluorescent dye was injected into a cell located in the center of cell colonies, followed by counting number of cells to which the dye was transferred. The results showed that ECCD-1 inhibits the dye transfer between cells, suggesting that the Ca2+-dependent cell-to-cell adhesion system (CDS) is essential for the functions of gap junction. |
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Keywords: | To whom offprint requests should be sent. |
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