Leishmania mexicana: Purine metabolism in promastigotes,axenic amastigotes,and amastigotes derived from vero cells |
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| Authors: | Brian D Hansen HKyle Webster Larry D Hendricks Michael G Pappas |
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| Institution: | 1. Division of Biochemistry Walter Reed Army Institute of Research, Washington, D.C. 20307, USA;2. Division of Medicine Walter Reed Army Institute of Research, Washington, D.C. 20307, USA;3. Division of Experimental Therapeutics Walter Reed Army Institute of Research, Washington, D.C. 20307, USA;4. Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20307, USA |
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| Abstract: | Leishmania mexicana mexicana promastigotes, axenic amastigotes, and amastigotes derived from Vero cells were examined for de novo purine synthesis and mechanisms of purine salvage. Both promastigotes and axenic amastigotes were incapable of de novo purine synthesis, as shown by the lack of 14C]formate and 14C]glycine incorporation into purine nucleotide pools. However, the ready incorporation of 14C]hypoxanthine, 14C]adenine, and 14C]guanine suggested that purine salvage pathways were operating. In addition, a significant percentage (?60%) of the total label from these purine precursors was associated with adenylate nucleotides. Nucleotide pool levels of axenic amastigotes were consistently greater but the specific activities were less than those of promastigotes, suggesting a slower rate of purine metabolism in the axenic amastigote form. Similar results were obtained from amastigotes isolated from infected Vero cells. |
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| Keywords: | Protozoa parasitic Hemoflagellate Promastigote Amastigote axenic and derived from Vero cells Purine synthesis Purine salvage pathways Purine nucleotides Adenylate nucleotides Guanylate nucleotides Nucleotide pool Chromatography high-pressure liquid |
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