Intermediate states between P* and Pf in bacterial reaction centers,as detected by the fluorescence kinetics |
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Authors: | Pierre Sebban Jean Claude Barbet |
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Affiliation: | Laboratoire de Photosynthese, Centre National de la Recherche Scientifique BP 1, 91190 Gif-sur-Yvette, France;Laboratoire pour l''Utilisation du Rayonnement Electromagnetique, C.N.R.S., Bat 209 C, 91405 Orsay Cedex, France |
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Abstract: | The fluorescence lifetimes of the reaction centers isolated from the wild-type Rhodopseudomonas sphaeroides purple bacterium and those from the R26 mutant strain, lacking the carotenoid, were measured at low redox potential. In addition to the prompt fluorescence occurring directly from P* and the long delayed emission related to the radical pair state Pf, two other components are present. We suggest that they may come from intermediate states between P* and Pf, or reflect the stabilization of Pf itself. |
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Keywords: | Photosynthesis Primary reaction Purple bacterium Reaction center Fluorescence lifetime Phase fluorimetry LDAO, lauryldimethylamine oxide BPh, bacteriopheophytin BChl, bacteriochlorophyll |
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