Product inhibition in simultaneous saccharification and fermentation of cellulose into lactic acid |
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Authors: | Prashant V Iyer YY Lee |
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Institution: | (1) Chemical Engineering Department, Auburn University, Auburn, AL 36849, USA |
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Abstract: | The product, lactic acid, strongly inhibited microbial activity in lactic acid fermentation. The volumetric productivity declined from 1.19 g/l.h with zero lactic acid (control) to only 0.18 g/l.h when lactic acid reached 65 g/l. Lactic acid also inhibited cellulase activity but less severely than the inhibition on microbial activity as lactic acid above 90 g/l was needed for 50% inhibition. A gradual deterioration of the Simultaneous Saccharification and Fermentation (SSF) process occurred with the build-up of lactic acid and the rate-controlling step in SSF shifted from hydrolysis to fermentation as the bioprocess proceeded. |
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Keywords: | enzymatic hydrolysis lactic acid product inhibition simultaneous saccharification and fermentation |
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