| 利用基于PCR的分子标记区分普通小麦-提莫菲维小麦渐渗系 |
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| 引用本文： | 纪剑辉,曹爱忠,王海燕,覃碧,王苏玲,孔芳,陈佩度,刘大钧,王秀娥. 利用基于PCR的分子标记区分普通小麦-提莫菲维小麦渐渗系[J]. 遗传, 2007, 29(10): 1256-1262. DOI: 10.1360/yc-007-1256 |
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| 作者姓名： | 纪剑辉 曹爱忠 王海燕 覃碧 王苏玲 孔芳 陈佩度 刘大钧 王秀娥 |
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| 作者单位： | 南京农业大学作物遗传与种质创新国家重点实验室细胞遗传所, 南京 210095 |
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| 基金项目： | 教育部高等学校博士学科点专项科研基金，国家高技术研究发展计划(863计划)，教育部长江学者奖励计划，教育部科学技术研究项目 |
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| 摘 要： | 采用定位于小麦2B染色体上的72对分子标记对含小麦抗白粉病基因Pm6的8份普通小麦（T.aestivum L.）-提莫菲维（T.timopheevii zhuk.）渐渗系材料进行分析, 通过分子标记标图确定8份材料中渗入的提莫菲维小麦染色体片段的大小, 同时结合连锁图谱对这些材料进行了遗传和物理标图。参考本研究所用的分子标记在染色体2B上的定位结果, Pm6基因被位于2B 染色体长臂近末端2BL-6区域, 提莫菲维小麦2G染色体渐渗片断长度由短到长排列顺序为: IGV1-465
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| 关 键 词： | Pm6 白粉病 渐渗系 提莫菲维小麦 小麦 |
| 文章编号： | 10.1360/yc-007-1256 |
| 收稿时间： | 2007-01-29 |
| 修稿时间： | 2007-01-29 |
Discrimination of the Triticum aestivum-T. timopheevii introgression lines using PCR-based molecular markers |
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| JI Jian-Hui,CAO Ai-Zhong,WANG Hai-Yan,QIN Bi,WANG Su-Ling,KONG Fang,CHEN Pei-Du,LIU Da-Jun,WANG Xiu-E. Discrimination of the Triticum aestivum-T. timopheevii introgression lines using PCR-based molecular markers[J]. Hereditas, 2007, 29(10): 1256-1262. DOI: 10.1360/yc-007-1256 |
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| Authors: | JI Jian-Hui CAO Ai-Zhong WANG Hai-Yan QIN Bi WANG Su-Ling KONG Fang CHEN Pei-Du LIU Da-Jun WANG Xiu-E |
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| Affiliation: | State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | In order to determine the fragment size of Triticum timopheevii chromosome segments introduced into wheat background and physically map the Pm6 gene, a total of 72 primers (including SSR and STS primers) were used to analyze the eight introduced introgression lines containing Pm6 gene. Referring to the available mapping information of the ana- lyzed markers on chromosome 2B, Pm6 was physically located in distal part of the long arm of chromosome 2B at the re- gion of Bin 2BL-6. The introgressed fragment sizes of the chromosome 2G in different introgression lines was determined to be as follow (from short to long): IGV1-465
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| Keywords: | Pm6 |
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