| 水蛭素12肽与低分子量单链尿激酶融合基因的构建、表达及产物特性分析 |
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| 引用本文： | 刘凤云,李秀珍,李风知,程度胜.水蛭素12肽与低分子量单链尿激酶融合基因的构建、表达及产物特性分析[J].生物技术通讯,1996(3). |
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| 作者姓名： | 刘凤云 李秀珍 李风知 程度胜 |
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| 作者单位： | 军事医学科学院生物工程研究所，军事医学科学院生物工程研究所，军事医学科学院生物工程研究所，军事医学科学院生物工程研究所 北京 100071，北京 100071，北京 100071，北京 100071 |
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| 摘 要： | 用化学合成的方法合成了水蛭素12肽基因的编码序列,通过DNA重组技术将水蛭素12肽基因片段与低分子量单链尿激酶cDNA片段连接构建了融合基因。融合基因在大肠杆菌中获得表达。体外实验结果表明,表达的融合蛋白具有溶纤活性和抗凝活性。
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| 关 键 词： | 水蛭素12肽 低分子量单链尿激酶 溶纤活性 抗凝活性 |
Consturction, Expression and Characterization of the Hybrid Protein Which Fused Peptide 12 Derived from Hirudin to Low Molecular Weight Single-Chain Urokinase |
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| Liu Fengyun,Li Xiuzhen,Li Fengzhi et al.Consturction, Expression and Characterization of the Hybrid Protein Which Fused Peptide 12 Derived from Hirudin to Low Molecular Weight Single-Chain Urokinase[J].Letters in Biotechnology,1996(3). |
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| Authors: | Liu Fengyun Li Xiuzhen Li Fengzhi |
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| Abstract: | A hybrid protein was constructed by fusion of the 12aa of the COOH-terminal of hirudin to the NH2-terminal of low molecular weight single-chain urokinase -type plasminogen activator. We have expressed the hybrid protein in Eschericliia colt and proved it has both fibrinolytic activity and anticoagulant activity. |
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| Keywords: | Peptide 12 derived from Hirudin Low molecular weight single-chain urokinase Fibrinolytic activity Anticoagulant activity |
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