Pluripotent differentiation of single F9 embryonal carcinoma cells |
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| Authors: | Peter Koopman Richard G. H. Cotton |
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| Affiliation: | 1. Department of Molecular Medicine and Personalized Therapeutics, Department of Biochemistry, Molecular Biology, Nutrition, and Metabolism, University Hospital of Nancy, F-54000, France;2. INSERM, U1256, NGERE – Nutrition, Genetics, and Environmental Risk Exposure, University of Lorraine, Nancy F-54000, France;3. First Department of Internal Medicine, Martin Luther University Halle-Wittenberg, Ernst-Grube-Str. 40, 06120 Halle (Saale), Germany;4. Department of Hepatology and Gastroenterology, University Hospital of Nancy, Nancy F-54000, France;5. ESPRI-BioBase Unit, Methodological and Biostatistical Support Unit, Platform of Clinical Research Support PARC, University Hospital of Nancy, Nancy F-54000, France;6. Department of Digestive, Hepatobiliary and Endocrine Surgery, University Hospital of Nancy, Nancy, F-54000, France;7. Department of Radiology, University Hospital of Nancy, Nancy, F-54000, France;8. Division of Gastroenterology and Rheumatology, Department of Medicine, Dermatology and Neurology, University of Leipzig, Liebigstraße 20, D-04103 Leipzig, Germany |
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| Abstract: | F9 embryonal carcinoma cells were induced to form a variety of differentiated cell types in monolayer culture. Cells with the morphological, histochemical and immunocytochemical properties of parietal and visceral endoderm, neurones and adipocytes were identified. Cells expressing Thy-1 antigen and large, multinucleated cells expressing cytoplasmic fibronectin were also observed. Various cell types were found together in colonies derived from individual F9 cells, allowing us to conclude that F9 cells are pluripotent in vitro. |
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