CFD analysis of the turbulent flow in baffled shake flasks |
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| Institution: | 1. DSM Biotechnology Center, Alexander Fleminglaan 1, 2613 AX Delft, Netherlands;2. Transport Phenomena, Chemical Engineering Department, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, Netherlands;3. Bioprocess Engineering, Biotechnology Department, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, Netherlands;1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;2. Dalian Practical Biotechnology Co., Ltd., Dalian, China;3. Department of Chemical Engineering, Delft University of Technology, Julianalaan 136, 2628 BL Delft, The Netherlands;1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;2. Department of Bioengineering, Imperial College London, London SW7 2AZ, UK;3. Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, UK;1. Aalto University, School of Chemical Engineering, P.O. Box 16100, 00076 Aalto, Finland;2. University of Chemistry and Technology in Prague, Technicka 5, 166 28 Prague, Czech Republic;1. Pharmaceutical Production Research Facility, Schulich School of Engineering, University of Calgary, 2500 University Dr. NW, Calgary, AB, T2N 1N4, Canada;2. Biomedical Engineering Graduate Program, University of Calgary, 2500 University Dr. NW, Calgary, AB, T2N 1N4, Canada;3. Department of Chemical and Petroleum Engineering, Schulich School of Engineering, University of Calgary, 2500 University Dr. NW, Calgary, AB, T2N 1N4, Canada;4. Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, 3330 Hospital Dr. NW, Calgary, AB, T2N 4N1, Canada;1. CNRS Laboratoire Réactions et Génie des Procédés, UMR 7274, 2 avenue de la forêt de Haye TSA 40602, Vandoeuvre-les-Nancy, F-54518, France;2. Université de Lorraine, LRGP, CNRS UMR 7274, 2 avenue de la forêt de Haye TSA 40602, Vandoeuvre-les-Nancy, F-54518, France;3. CNRS, Laboratoire de Génie Chimique (LGC UMR 5503), 4 allée Emile Monso, BP 84234, F-31432 Toulouse, France;4. Université de Toulouse, INPT, ENSIACET, F-31432 Toulouse, France;5. INRA, Laboratoire PIHM UR 638, BP 39, F-59651 Villeneuve d?Ascq, France |
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| Abstract: | In this work, computational fluid dynamics (CFD) technique is used to simulate the complicated unsteady-state turbulent flow field formed in baffled flask. The baffled flask shows advantages both in mass transfer capacity and in shear formation in comparison with unbaffled flasks. Detailed investigations of power consumption, mass transfer and shear rate are carried out in baffled flasks under shaking frequencies ranging from 100 rpm to 250 rpm, and filling volumes from 50 mL to 150 mL. The results show that the specific power input and specific interface area are both greatly influenced by shaking frequency and filling volume. For the positive effect of shaking frequency on both mass transfer coefficient (kL) and specific interface area (a), the volumetric mass transfer coefficient (kLa) increases greatly with shaking frequency. Results also show that filling volume has no significant effect on kL but negative effect on specific interface area. Shear force formed in baffled flask shows great dependent on shaking frequency, but it is insensitive to the filling volume. Based on these investigations, correlations linking these parameters are proposed. Finally, cultivations of filamentous fungus conducted in unbaffled and baffled flasks validated the simulating results. |
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