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Sampling location of the inoculum is crucial in designing anodes for microbial fuel cells
Affiliation:1. Centre Technique du Papier, BP 251, 38044 Grenoble Cedex 9, France;2. Laboratoire de Génie Chimique (LGC), CNRS-Université de Toulouse (INPT), BP 84234, 31432 Toulouse, France;3. Laboratoire d’Ecologie Microbienne de la Rhizosphère et d’Environnements Extrêmes (LEMiRE), UMR 6191, CNRS-CEA-Aix-Marseille Université, CEA/DSV/iBEB, CEA Cadarache, 13108 Saint Paul Lez Durance, France;1. CEA, Division of Immuno-Virology, IDMIT Center, iMETI/DSV, Fontenay-aux-Roses, France;2. UMR-1184, Université Paris Sud 11, Orsay, France;3. Vaccine Research Institute (VRI), Créteil, France;4. INSERM, Paris, France;5. Bertin Pharma, Montigny-Le-Bretonneux, France;6. INSERM, Unite U955, Créteil F-94010, France;7. Université Paris-Est, Faculté de Médecine, Hôpital Albert-Chenevier, Créteil F-94010, France;8. Hôpital Henri-Mondor, Service d’Immunologie clinique, UMR-S 955, Créteil F-94010, France;1. Department of Electrical Engineering, Centro Universitário da FEI, São Bernardo do Campo, Brazil;2. Département des Composants Silicium – SCME/LCTE, CEA-LETI Minatec, Grenoble, France;1. Institut Curie Centre de Protonthérapie d''Orsay, Centre Universitaire, Orsay, France;2. Institut Curie, Centre Universitaire, Orsay, France;3. INSERM U612, Centre Universitaire, Orsay, France;4. Centre d''Etude Atomique, Direction des Sciences du Vivant, Institut des Maladies Emergentes et des Thérapies Innovantes, Service d''Etude des Prions et des Infections Atypiques, Fontenay-aux-Roses, France;1. Institute of Psychological Medicine and Clinical Neurosciences, Cardiff University, Hadyn Ellis Building, Maindy Road, Cardiff, CF24 4HQ;2. Inserm U744, Lille, 59000, France;3. Université Lille 2, Lille, 59000, France;4. Institut Pasteur de Lille, Lille, 59000, France;5. Department of Epidemiology, Clinical Genetics and Neurology, Erasmus MC University Medical Center, Rotterdam, 3015 CE, The Netherlands;6. The John P. Hussman Institute for Human Genomics, University of Miami, Miami, FL, 33124, USA;7. Department of Medicine (Biomedical Genetics), Boston University School of Medicine, Boston, MA, 02218, USA;8. Department of Ophthalmology, Boston University School of Medicine, Boston, MA, 02215, USA;9. Department of Biostatistics, Boston University School of Public Health, Boston, MA, 02118, USA;10. Cardiovascular Health Research Unit, Department of Medicine, University of Washington, Seattle, WA, 98101, USA;11. Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami, Miami, FL, 33124, USA;12. Functional Genomics Center, Zurich, 8038, Switzerland;13. Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37212, USA;14. University of Iceland, Faculty of Pharmaceutical Sciences, Reykjavik, 525-4000, Iceland;15. Icelandic Heart Association, Kopavogur, 525-4000, Iceland;p. Departments of Epidemiology, Neurology and Radiology, Erasmus MC University Medical Center, Rotterdam, 3015 CE, The Netherlands;q. Netherlands Consortium for Healthy Aging, Leiden, The Netherlands;r. Centre National de Genotypage, Institut Genomique, Commissariat à l''énergie Atomique, Evry, 91300, France;s. Fondation Jean Dausset- CEPH, Paris, 75010, France;t. Department of Pathology and Laboratory Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104, USA;u. Institute for Molecular Biology and Biochemistry, Medical University of Graz, Graz, 8010, Austria;v. Memory Clinic of Fundació ACE. Institut Català de Neurociències Aplicades, Barcelona, 8029, Spain;w. Taub Institute on Alzheimer''s Disease and the Aging Brain, Department of Neurology, Columbia University New York, NY, 10027, USA;x. Gertrude H. Sergievsky Center, Columbia University, Department of Neurology, Columbia University, New York, NY, 10027, USA;y. Centre Hospitalier Régional Universitaire de Lille, Lille, 59000, France;z. Department of Psychiatry and Psychotherapy and Institute of Human Genetics, University of Bonn, Bonn, 53113, Germany;11. UMR 894 Inserm, Faculté de Médecine, Université Paris Descartes, Paris, 75014, France;22. Departments of Epidemiology and Global Health, University of Washington, Seattle, WA, 98101, USA;33. Department of Neuroscience, Mount Sinai School of Medicine, New York, NY, 10027, USA;44. Department of Psychiatry, Mount Sinai School of Medicine, New York, NY, 10027, USA;55. Departments of Genetics and Genomic Sciences, Mount Sinai School of Medicine, New York, NY, 10027, USA;66. INSERM U614, Faculté de Médecine, Centre Hospitalier du Rouvray, Rouen, 76000, France;77. Department of Medicine, University of Washington, Seattle, WA, 98195, USA;88. German Center for Neurodegenerative Diseases (DZNE, Bonn) and Institute for Medical Biometry, Informatics and Epidemiology, University of Bonn, Bonn, Germany, 53113;99. Department of Psychiatry and Hope Center Program on Protein Aggregation and Neurodegeneration, Washington University School of Medicine, St. Louis, MO, 98122, USA;1010. Ageing Group, Centre for Public Health, School of Medicine, Dentistry and Biomedical Sciences, Queen''s University Belfast, BT7 1NN, UK;1111. Department of Epidemiology, Erasmus MC University Medical Center, Rotterdam, 3015 CE, The Netherlands;1212. INSERM U888, Hôpital La Colombière, Montpellier, 34000, France;1313. Departments of Neurology, University of Pittsburgh School of Medicine, Pittsburgh, PA, 15213, USA;1414. Program in Translational NeuroPsychiatric Genomics, Institute for the Neurosciences, Department of Neurology & Psychiatry, Brigham and Women''s Hospital and Harvard Medical School, Boston, MA, 02215, USA;1515. Program in Medical and Population Genetics, Broad Institute, Boston, MA, 02215, USA;pp. Rush Institute for Healthy Aging, Department of Internal Medicine, Rush University Medical Center, Chicago, IL, 60612, USA;qq. King''s College London, Institute of Psychiatry, Department of Neuroscience, De Crespigny Park, Denmark Hill, London SE5;rr. Inserm U897, Victor Segalen University, F-33076, Bordeaux, 33000, France;ss. Department of Psychiatry and Psychotherapy, Friedrich-Alexander-University Erlangen-Nuremberg, Germany;tt. Cambridge Institute for Medical Research, University of Cambridge, Cambridge, CB2 OXY, UK;uu. Neurodegenerative Brain Diseases Group, Department of Molecular Genetics, VIB, Antwerp, 2000, Belgium;vv. Laboratory of Neurogenetics, Institute Born-Bunge, University of Antwerp, Antwerp, 2000, Belgium;ww. Neurogenomics Division, Translational Genomics Research Institute, Phoenix, AZ, 85004, USA;xx. Mercer''s Institute for Research on Aging, St. James Hospital and Trinity College, Dublin, Dublin 8, Ireland;yy. Institute of Genetics, Queen''s Medical Centre, University of Nottingham, NG7 2UH, UK;zz. Department of Human Genetics, University of Pittsburgh, Pittsburgh, PA, 15213, USA;111. Alzheimer''s Disease Research Center, University of Pittsburgh, Pittsburgh, PA, 15213, USA;222. Aging Reasearch Center, Dept Neurobiology, Care Sciences and Society, Karolinska Institutet and Stockholm University, Stockholm, 17177, Sweden;333. Group Health Research Institute, Group Health, Seattle, WA, 98195, USA;444. Department of Psychiatry and Behavioral Sciences, Miller School of Medicine, University of Miami, Miami, FL, 33124, USA;555. University of Bristol, Clinical Neurosciences, Level 1 Learning and Research Building BS10 5NB;666. Tanz Centre for Research in Neurodegenerative Disease, University of Toronto, Toronto, ON M5S 1A1, Canada;777. Institute of Primary Care and Public Health, Cardiff University, Neuadd Meirionnydd, University Hospital of Wales, Heath Park, Cardiff, CF14 4XN, UK;888. Cambridge Institute for Medical Research and Department of Clinical Neurosciences, University of Cambridge, Cambridge, CB2 1TN, UK;999. Neurology Department, IIB Sant Pau, Sant Pau Hospital, Universitat Autònoma de Barcelona, Barcelona, 08026, Spain;101010. Center for Networker Biomedical Research in Neurodegenerative Diseases (CIBERNED), Barcelona, Spain;111111. Department of Psychiatry and Behavioral Sciences, University of Washington, Seattle, WA, 98195, USA;121212. Department of Neurological Sciences, Rush University Medical Center, Chicago, IL, 60612, USA;131313. Aristotle University of Thessaloniki, Despere 3, Thessaloniki, 54621, Greece;141414. Clinical and Behavioral Neurology, Fondazione Santa Lucia, Roma, 00142, Italy;151515. MRC Prion Unit, Department of Neurodegenerative Disease, UCL Institute of Neurology, London, WC1N 3BG, UK;ppp. Department of Neurological and Psychiatric Sciences, University of Florence, Florence, 50121, Italy;qqq. Centro di Ricerca, Trasferimento e Alta Formazione DENOTHE, University of Florence, Florence, Italy;rrr. Department of Immunology, Hospital Universitario Dr. Negrin, Las Palmas de Gran Canaria, 35012, Spain;sss. Dementia Research Centre, Department of Neurodegenerative Disease, UCL Institute of Neurology, London, WC1N 3BG, UK;ttt. Department of Molecular Neuroscience and Reta Lilla Weston Laboratories, Institute of Neurology, London, WC1N 3BG, UK;uuu. IRCCS Associazione Oasi Maria SS, Troina, 94018, Italy;vvv. Oxford Healthy Aging Project (OHAP), Clinical Trial Service Unit, University of Oxford, Oxford, OX1 2JD, UK;www. Cognitive Function and Ageing Study (CFAS), Institute of Public Health, University of Cambridge, Cambridge, CB2 1TN, UK;xxx. University of Milan, Fondazione Cà Granda, IRCCS Ospedale Policlinico, Milan, 20122, Italy;yyy. Neurological Clinic, University of Pisa, 56126, Italy;zzz. Urban Epidemiology, Institute for Medical Informatics, Biometry and Epidemiology, University Hospital Essen, University Duisburg-Essen, Essen, 45141, Germany;1111. Section of Gerontology and Geriatrics, Department of Clinical and Experimental Medicine, University of Perugia, Perugia, 06100, Italy;2222. Section of Neuroscience and Clinical Pharmacology, Department of Biomedical Sciences, University of Cagliari, Cagliari, 09123, Italy;3333. Department of Psychiatry and Psychotherapy, University of Bonn, Germany and German Center for Neurodegenerative Diseases (DZNE, Bonn), Bonn, 53113, Germany;4444. Université Pierre et Marie Curie, Département de Neurologie, Institut de la Mémoire et de la Maladie d’Alzheimer, Paris, France;5555. Gerontology and Geriatrics Research Laboratory, I.R.C.C.S. Casa Sollievo della Sofferenza, San Giovanni Rotondo (FG), 71013, Italy;6666. Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Madrid, 28046, Spain;7777. Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Madrid, Spain;8888. Instituto de Investigación Sanitaria “Hospital la Paz” (IdIPaz), Madrid, Spain;9999. Departement of Geriatrics, Center for Aging Brain, University of Bari, Bari, 70121, Italy;10101010. Department of Neuroscience-University of Parma, Parma, 43100, Italy;11111111. Center for Cognitive Disorders AUSL, Parma, Italy;12121212. Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami, Miami, FL, 33124, USA;13131313. Dept. Of Psychiatry, University Hospital, 66424, Saarland;14141414. Dept. of Public Health/Geriatrics, Uppsala University, Uppsala, 75312, Sweden;15151515. Center for Applied Genomics, Children''s Hospital of Philadelphia, Philadelphia, PA, 19104, USA;pppp. Department of Neuroscience, Mayo Clinic, Jacksonville, FL, 32224, USA;qqqq. National Alzheimer''s Coordinating Center, University of Washington, Seattle, WA, 98195, USA;rrrr. Genetica molecular-Huca-Oviedo, Oviedo, 33006, Spain;ssss. Neurology Service and CIBERNED, “Marqués de Valdecilla” University Hospital (University of Cantabria and IFIMAV), Santander, 39005, Spain;tttt. Department of Medical and Molecular Genetics, Indiana University, Indianapolis, IN, 46202, USA;uuuu. Landspitali University Hospital, Reykjavik, 543-1000, Iceland;vvvv. Institute of Clinical Medicine - Neurology, University of Eastern Finland, Kuopio, 70211, Finland;wwww. Department of Neurology, Kuopio University Hospital, FIN-70211, Kuopio, Finland;xxxx. Department of Epidemiology, Harvard School of Public Health, Boston, MA, 02115, USA;yyyy. Department of Epidemiology, Boston University School of Public Health, Boston, MA, 02118, USA;zzzz. Department of Psychiatry, Massachusetts General Hospital/Harvard Medical School, Boston, MA, 02115, USA;11111. Department of Medicine (Geriatrics), University of Mississippi Medical Center, Jackson, MS, 39216, USA;22222. Rush Alzheimer''s Disease Center, Rush University Medical Center, Chicago, IL, 60612, USA;33333. Laboratory of Epidemiology, Demography, and Biometry, National Institute of Health, Bethesda, MD, 20892, USA;44444. Aging Reasearch Center, Dept Neurobiology, Care Sciences and Society, Karolinska Institutet and Stockholm University, Stockholm, 11418, Sweden;55555. Dept Geriatric Medicine, Genetics Unit, Karolinska University Hospital Huddinge, S-14186, Stockholm;66666. Division of Clinical Neurosciences, School of Medicine, University of Southampton, Southampton, SO17 1BJ, UK;77777. Departments of Neurology and Epidemiology, Erasmus MC University Medical Center, Rotterdam, 3015 CE, The Netherlands;88888. Department of Pathology, University of Washington, Seattle, WA, 98195, USA;99999. Medical Genetics Institute, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA;1010101010. CRicm-UMRS975, Paris, France;1111111111. AP-HP, Hôpital de la Pitié-Salpêtrière, Paris, 75013, France;1212121212. Department of Epidemiology, University of Washington, Seattle, WA, 98195, USA;1313131313. Laboratory of Neurogenetics, Intramural Research Program, National Institute on Aging, Bethesda, MD, 20892, USA;1414141414. Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX, 77030, USA;1515151515. Human Genetics Center and Div. of Epidemiology, University of Texas Health Sciences Center at Houston, Houston, TX, 77030, USA;ppppp. Department of Psychiatry, University Hospital, 66424, Saarland;qqqqq. Memory Clinic of Fundació ACE, Institut Català de Neurociències Aplicades, Barcelona, 8029, Spain;rrrrr. Department of Neurology, Medical University of Graz, Graz, 8010, Austria;sssss. Department of Psychiatry, University of Munich, Munich and University of Halle, Halle, 80336, Germany;ttttt. Centre de Mémoire de Ressources et de Recherche de Bordeaux, CHU de Bordeaux, Bordeaux, France;uuuuu. Inserm U708, Victor Segalen University, F-33076, Bordeaux, 33000, France;vvvvv. Institute of Human Genetics, Department of Genomics, Life and Brain Center, University of Bonn, and German Center for Neurodegenerative Diseases (DZNE, Bonn), Bonn, 53113, Germany;wwwww. Karolinska Institutet, Dept Neurobiology, Care Sciences and Society, KIADRC, Novum floor 5, S14186 Stockholm, 17177, Sweden;xxxxx. Vanderbilt Center for Human Genetics Research, Vanderbilt University, Nashville, TN, 37212, USA;yyyyy. McGill University and Génome Québec Innovation Centre, Montreal, Canada;zzzzz. Department of Neurology, Boston University School of Medicine, Boston, MA, 02215, USA;111111. Neurogenetics Laboratory, Division of Neurosciences, Center for Applied Medical Research, University of Navarra School of Medicine, Pamplona, 28023, Spain;222222. Neurogenetics Laboratory, Division of Neurosciences, Center for Applied Medical Research and Department of Neurology, Clínica Universidad de Navarra, University of Navarra School of Medicine, Pamplona, Spain;333333. CIBERNED, Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas, Instituto de Salud Carlos III, Spain;444444. Institute for Stroke and Dementia Research, Klinikum der Universität München, Munich, Germany and German Center for Neurodegenerative Diseases (DZNE, Munich), Munich, 80336, Germany;555555. Departments of Epidemiology and Health Services, University of Washington, Seattle, WA, USA, Group Health Research Institute, Group Health, Seattle, WA, USA;666666. Neuropsychiatric Genetics Group, Department of Psychiatry, Trinity Centre for Health Sciences, St James''s Hospital, Dublin 8;1. SQPOV, UMR0408, Avignon Université, INRA, F-84914 Avignon, France;2. CEA, DRF/Joliot/DMTS/SPI, Li2D, Laboratory "Innovative technologies for Detection and Diagnostics", Bagnols-sur-Cèze F-30200, France;1. Radiopathology Laboratory, Autoridad Regulatoria Nuclear (ARN), Buenos Aires, Argentina;2. Toxicology Laboratory, Universidad Nacional del Sur (UNS), Bahía Blanca, Argentina;3. Commissariat a l’Energie Atomique et aux Energies Alternatives (CEA), Institute of Emerging Diseases and Innovative Therapies (iMETI), Research Division in Hematology and Immunology (SRHI), Paris, France;4. University Paris Diderot, Sorbonne Paris Cité, UMR E-5 Institut Universitaire d’Hematologie, Saint-Louis Hospital, Paris, France
Abstract:A Kraft pulp mill effluent was used as the inoculum to form microbial bioanodes under controlled potential at +0.4 V/SCE. Samples were collected at the inlet and outlet of the aerated lagoon of the treatment line. The outlet sample allowed efficient bioanodes to be designed (5.1 A/m2), which included Geobacter and Desulfuromonas sp. in their microbial community. In contrast, the bioanodes formed with the inlet sample did not contain directly connecting anode-respiring bacteria and led to lower currents. It was necessary to re-form this bioanode at lower applied potential (−0.2 V/SCE) to select more efficient electroactive species and increase the current density to 5 A/m2.
Keywords:
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