Covalent immobilization of hydroperoxide lyase on chitosan hybrid hydrogels and production of C6 aldehydes by immobilized enzyme |
| |
| Affiliation: | 1. Department of General Surgery, Yichang Central People''s Hospital and The First Affiliated Hospital of China Three Gorges University, 443002 Yichang, People''s Republic of China;2. Department of Biochemistry and Molecular biology, College of Basic Medicine, Wuhan University, Wuhan 430071, People''s Republic of China;3. Institute of Molecular Biology of Three Gorges University, Yichang, People''s Republic of China;1. National Engineering Laboratory for Electric Cehicles, Beijing Institute of Technology,Beijing 100081, China;2. Collaborative Innovation Center of Electric Vehicles in Beijing, Beijing Institute of Technology, Beijing 100081, China;1. State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Centre for Bio Therapy, Chengdu 610041, China;2. Advanced Bioprocessing Centre, College of Engineering, Design and Physical Sciences, Brunel University London, Uxbridge, UB8 3PH, UK;3. Department of Pharmacology and Pharmaceutical Sciences, School of Medicine, Tsinghua University and Collaborative Innovation Centre for Biotherapy, Beijing 100084, China;1. Department of Physics, Cotton University, Guwahati, 781001, India;2. Department of Physics and Electronics, Rhodes University, P O Box 94, Grahamstown, 6140, South Africa;1. Dubrava University Hospital, Zagreb, Croatia;2. University Hospital, Zagreb, Croatia;3. University of Zagreb Medical School, Croatia;1. Institute of Mechanics and Graphic Arts, Faculty of Production Engineering, Warsaw University of Technology, ul. Konwiktorska 2, Warsaw 00-217, Poland;2. Department of Manufacturing Engineering, Faculty of Mechanical Engineering and Design, Kaunas University of Technology, Studentų 56, Kaunas 51424, Lithuania |
| |
| Abstract: | Five kinds of spacer arm attached chitosan hybrid hydrogels were tested for the possibility of being used as carriers for the immobilization of hydroperoxide lyase from amaranthus tricolor leaves. The 1,6-hexamethylenediamine attached chitosan-κ-carrageenan with biomimetic hydrophobic surface was proved to be the most suitable carrier. A maximum activity of 7.49 ± 0.19 U/g and a yield of 95% were obtained under optimized coupling condition. Meanwhile, the affinity between enzyme and substrates was not reduced after immobilization, as evidenced by the fact that the Km value of hydroperoxide lyase decreased from 108.6 to 79.97 μM for 13-hydroperoxy-linoleic-acid and almost unchanged for 13-hydroperoxy-linolenic-acid. Furthermore, the thermal, operational and storage stabilities of HPL were significantly improved after immobilization. Using the immobilized enzyme as the catalyst, the yield of 2(E)-hexenal and hexanal reached 1374.8 ± 51.8 mg/L and 1987.9 ± 67.9 mg/L, respectively, and the amount of immobilized enzyme needed in the reaction mixture was much lower than its free counterpart. |
| |
| Keywords: | Immobilization Hydroperoxide lyase Amaranthus tricolor Hybrid chitosan hydrogel Spacer arm |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
