Three-step pathway engineering results in more incidence rate and higher emission of nerolidol and improved attraction of Diadegma semiclausum |
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| Institution: | 1. Laboratory of Plant Physiology, Wageningen University, PO Box 658, 6700 AR Wageningen, The Netherlands;2. Microbiologia, Departament de Biologia, Univeristat de les Illes Balears, Palma de Mallorca, Spain;3. Department of Molecular Genetics, Center for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Campus UAB, Bellaterra (Cerdanyola del Vallès) and, Departament de Bioquímica i Biologia Molecular, Facultat de Farmàcia, Universitat de Barcelona, Barcelona, Spain;4. Laboratory of Entomology, Wageningen University, PO Box 8031, 6700 EH Wageningen, The Netherlands;1. Facultat de Farmàcia and Institut de Biomedicina, Universitat de Barcelona, Av. Joan XXIII, 27-31, 08028 Barcelona, Spain;2. Catalan Institution for Research and Advanced Studies (ICREA), Passeig Lluís Companys 23, 08010 Barcelona, Spain;3. YSBL, University of York, Heslington, York YO10 5DD, UK;4. Vernalis (R&D) Ltd, Granta Park, Abington, Cambridge CB21 6GB, UK;1. Institute for Sustainable Plant Protection, National Research Council, Portici (NA), Italy;2. Department of Life Sciences and Systems Biology, University of Turin, 10135 Turin, Italy;3. Department of Biological Science & Technology, Faculty of Industrial Science & Technology, Tokyo University of Science, Tokyo 125-8585, Japan |
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| Abstract: | The concentration and ratio of terpenoids in the headspace volatile blend of plants have a fundamental role in the communication of plants and insects. The sesquiterpene (E)-nerolidol is one of the important volatiles with effect on beneficial carnivores for biologic pest management in the field. To optimize de novo biosynthesis and reliable and uniform emission of (E)-nerolidol, we engineered different steps of the (E)-nerolidol biosynthesis pathway in Arabidopsis thaliana.Introduction of a mitochondrial nerolidol synthase gene mediates de novo emission of (E)-nerolidol and linalool. Co-expression of the mitochondrial FPS1 and cytosolic HMGR1 increased the number of emitting transgenic plants (incidence rate) and the emission rate of both volatiles. No association between the emission rate of transgenic volatiles and their growth inhibitory effect could be established. (E)-Nerolidol was to a large extent metabolized to non-volatile conjugates. |
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