An efficient biosurfactant-producing and crude-oil emulsifying bacterium Bacillus methylotrophicus USTBa isolated from petroleum reservoir |
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| Affiliation: | 1. School of Civil and Environmental Engineering, and National International Cooperation Based on Environment and Energy, University of Science and Technology Beijing, Beijing 100083, PR China;2. Key Laboratory of Biogeology and Environmental Geology of Chinese Ministry of Education, and Sino-Hungarian Joint Laboratory of Environmental Science and Health, University of Geoscience, Wuhan 430074, PR China;3. Department of Chemistry, Hong Kong Baptist University, 224 Waterloo Road, Kowloon Tong, Hong Kong Special Administrative Region;4. Department of Botany, Faculty of Science, University of Ruhuna, Matara, Sri Lanka;1. Department of Pharmaceutics, Gupta College of Technological Sciences, Asansol 713301, WB, India;2. Department of Pharmaceutics, Sinhgad Institute of Pharmacy, Narhe, Pune 411 041, India;3. Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China;1. Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran;2. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran;3. Faculty of Life & Health Sciences, University of Ulster, Coleraine BT52 1SA, N. Ireland, UK;4. Department of Medicinal Chemistry, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran;5. Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran;1. Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China;2. Unovation Bio & EP Technology Company Limited, Beijing, 100029, PR China;3. Research Institute of Petroleum Exploration & Development, PetroChina Company Limited, Beijing, 10083, PR China;4. Research Institute of Petroleum Exploration & Development, PetroChina Company Limited, Langfang, Hebei, 065007, PR China;5. School of Geosciences, Yangtze University, Wuhan, 430100, PR China;6. College of Chemistry & Environmental Engineering, Yangtze University, Wuhan, 430100, PR China;7. State Key Laboratory of Agrobiotechnology, Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing, 100193, PR China |
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| Abstract: | An efficient biosurfactant-producing bacterium was isolated and cultured from petroleum reservoir in northeast China. Isolate was screened for biosurfactant production using haemolytic assay, Cetyl Trimethyl Ammonium Bromide agar plate assay (CTAB) and the qualitative oil-displacement test. Based on partial sequenced 16S rDNA analysis of isolate, USTBa, identified as Bacillus methylotrophicus with 100% identity. This bacterium was able to produce a type of biosurfactant with excessive foam-forming properties. The maximum biosurfactant production was obtained when the cells were grown on minimal salt medium containing 2% (v/v) crude-oil as the sole source of carbon at 35 °C and 180 rpm after 192 h. This strain had a high emulsification activity and biosurfactant production of 78% and 1.8 g/L respectively. The cell free broth containing biosurfactant could reduce the surface tension to 28 mN/m. Fourier transform infrared (FT-IR) spectrum of extracted biosurfactant indicates the presence of carboxyl, hydroxyl and methoxyl functional groups. Elemental analysis of the biosurfactant by Energy dispersive X-ray spectroscopy (EDS) reveals that the biosurfactant was anionic in nature. The strain USTBa represented as a potent biosurfactant-producer and could be useful in variety of biotechnological and industrial processes, particularly oil industry. |
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