Carotenoid-based phenotypic screen of the yeast deletion collection reveals new genes with roles in isoprenoid production |
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| Institution: | 1. Joint BioEnergy Institute, Emeryville, CA 94608, USA;2. Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA;3. Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA;4. Department of Bioengineering, University of California, Berkeley, CA 94720, USA;1. Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia;2. Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt;3. Genetics and Cytology Department, Genetic Engineering and Biotechnology Division, National Research Center, Dokki, Egypt;4. Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Giza, Egypt;5. National Institute for Basic Biology, Myodaiji, Okazaki, Aichi 444-8585, Japan;1. Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA 94608, USA;2. Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA;3. Department of Bioengineering, University of California, Berkeley, CA 94720, USA;4. Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA;1. Joint BioEnergy Institute, 5885 Hollis St., Emeryville, CA 94608, USA;2. Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA;3. Department of Bioengineering and Biotechnology, Sandia National Laboratory, Livermore, CA, USA;4. Department of Bioengineering, University of California, Berkeley, CA, USA;5. Department of Chemical & Biomolecular Engineering, University of California, Berkeley, CA, USA;6. Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kogle Alle, DK2970 Hørsholm, Denmark;1. Center for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China;2. College of Biology Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China |
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| Abstract: | Beside their essential cellular functions, isoprenoids have value as pharmaceuticals, nutriceuticals, pesticides, and fuel alternatives. Engineering microorganisms for production of isoprenoids is relatively easy, sustainable, and cost effective in comparison to chemical synthesis or extraction from natural producers. We introduced genes encoding carotenoid biosynthetic enzymes into the haploid yeast deletion collection to identify gene deletions that improved isoprenoid production. Deletions that showed significant improvement in carotenoid production were further screened for production of bisabolene, an isoprenoid alternative to petroleum-derived diesel. Combining those deletions with other mevalonate pathway modifications increased production of bisabolene from 40 mg/L to 800 mg/L in shake-flask cultures. In a fermentation process, this engineered strain produced 5.2 g/L of bisabolene. |
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