Papaya fruit ripening: ROS metabolism,gene cloning,characterization and molecular docking of peroxidase |
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| Institution: | 1. Institute of Plant Biology, National Taiwan University, Taipei, Taiwan;2. Hualien District Agricultural Research and Extension Station, Council of Agriculture, Hualien, Taiwan;3. AVRDC-The World Vegetable Center, PO Box 42, Shanhua, Tainan 74199, Taiwan;4. Ecological Materials Technology Department, Green Energy & Eco-technology System Center, ITRI South Campus, Industrial Technology Research Institute, Tainan, Taiwan;1. The United Graduate School of Agricultural Science, Tottori University, Koyama-Minami, Tottori 680-8553, Japan;2. Faculty of Agriculture, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan |
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| Abstract: | Climacteric fruit ripening has been characterized by oxidative burst and involve active oxidative metabolism with generation of reactive oxygen species (ROS). In the present paper, the papaya fruit ripening was found to be associated with increase in polygalacturonase (PG), pectate lyase (PEL), catalase (CAT), ascorbate peroxidase (APX), H2O2 and lipid peroxidation concomitant with decrease in the activities of superoxide dismutase (SOD) and guaiacol peroxidase (GPX). Furthermore, a cDNA (903 bp) of GPX from unripe papaya fruit pulp was isolated and cloned. On BLAST analysis, the deduced protein exhibited homology with various peroxidases and specific hits for plant heme peroxidase family namely heme and calcium binding domains. GPX of papaya was modeled and docked with various substrates and inhibitors among which guaiacol and cysteine were found to be the best substrate and inhibitor, respectively. |
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| Keywords: | Anti-oxidant enzymes Fruit ripening Homology modeling Molecular docking Reactive oxygen species |
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