Effect of oxygen supply on biomass and helvolic acid production in submerged fermentation of Cordyceps taii |
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| Institution: | 1. State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;2. Division of Applied Mycology and Biochemical Pharmacy, Key Laboratory of Cell Engineering of Guizhou Province, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, China;3. State Key Laboratory of Microbial Metabolism, and Laboratory of Molecular Biochemical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai 200240, China;1. School of Engineering, University College Cork, Cork, Ireland;2. Department of Chemical Engineering, Autonomous University of Coahuila. Saltillo, Coahuila, Mexico;3. Biotechnology Department, Autonomous University of Coahuila. Saltillo, Coahuila, Mexico;4. Dipartimento di Scienze Biomediche, Odontoiatriche e delle Immagini Morfologiche e Funzionali, University of Messina, Messina, Italy;5. Dipartimento di Chimica e Tecnologie del Farmaco, Facoltà di Farmacia e Medicina, Sapienza Università di Roma, Roma, Italy;6. Chimie et Biotechnologie des Produits Naturels & ESIROI Agroalimentaire, Université de la Réunion, Ile de la Réunion, France;1. Department of Food Storage and Technology, Wroc?aw University of Environmental and Life Sciences, ul. Che?monskiego 37, 51-630 Wroc?aw, Poland;2. Department of Animal Nutrition and Feed Management, Wroc?aw University of Environmental and Life Sciences, ul. Che?monskiego 38c, 50-630 Wroc?aw, Poland;3. Swedish Centre for Resource Recovery, University of Borås, SE-50190 Borås, Sweden;1. R&D Department, GenChim Testing (Shanghai) Co., Ltd., Shanghai 200131, China;2. Key Laboratory of State Administration of Traditional Chinese Medicine, Sunshine Lake Pharma Co. Ltd., Guangdong 523850, China;3. Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;1. Department of Basic Pharmaceutical Sciences, School of Pharmacy, University of Louisiana at Monroe, 1800 Bienville Drive, Monroe, LA 71201, United States;2. Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Helwan, Cairo 11795, Egypt;3. Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, Scotland, UK;4. Pharmacognosy Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt;1. Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou 350014, China;2. National and Local Joint Engineering Research Center for Breeding & Cultivation of Featured Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou 350014, China |
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| Abstract: | The entomogenous fungus Cordyceps taii, a traditional Chinese medicinal mushroom, exhibits potent important pharmacological effects and it has great potential for health foods and medicine. In this work, the effects of oxygen supply on production of biomass and bioactive helvolic acid were studied in shake-flask fermentation of C. taii mycelia. The value of initial volumetric oxygen transfer coefficient (KLa) within 10.1–33.8 h?1 affected the cell growth, helvolic acid production and expression levels of biosynthetic genes. The highest cell concentration of 17.2 g/L was obtained at 14.3 h?1 of initial KLa. The highest helvolic acid production was 9.6 mg/L at 10.1 h?1 of initial KLa. The expression levels of three genes encoding hydroxymethylglutaryl-CoA synthase, hydroxymethylglutaryl-CoA reductase and squalene synthase were down-regulated on day 2 and day 8 but up-regulated on day 14 at an initial KLa value of 10.1 h?1 vs. 33.8 h?1, which well corresponded to the helvolic acid biosynthesis in those conditions. The information obtained would be helpful for improving the biomass and helvolic acid production in large-scale fermentation of C. taii. |
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| Keywords: | Fermentation Oxygen transfer Biosynthesis Filamentous fungi Biosynthetic gene expression Medicinal mushroom |
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