Stimulatory effects of Coix lacryma-jobi oil on the mycelial growth and metabolites biosynthesis by the submerged culture of Ganoderma lucidum |
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| Affiliation: | 1. School of Environmental and Life Sciences, Wenzhou University, Chashan University Town, Wenzhou 325035, China;2. Institute of Bioprocess Engineering, Wenzhou University, Chashan University Town, Wenzhou 325035, China;3. School of Food Science and Engineering, Jilin Agriculture University, Changchun 130018, China;1. Department of Physics, University of Oxford, Parks Road, Oxford OX1 3PU, UK;2. Atmospheric, Oceanic, Space Science, University of Michigan, 2455 Hayward St, Ann Arbor, MI 48103, USA;3. Plasma Physics Department, AWE Plc., Aldermaston, Reading RG7 4PR, UK;4. CFSA, Department of Physics, University of Warwick, Coventry CV4 7AL, UK;5. Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550, USA;6. SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA;7. ExtreMe Matter Institute, GSI Helmholtzzentrum für Schwerionenforschung GmbH, Planckstr. 1, 64291 Darmstadt, Germany;8. Max-Planck-Institut für Physik Komplexer Systeme, 01187 Dresden, Germany;9. Department of Physics, Imperial College London, SW7 2AZ, UK;1. College of Engineering, Mathematics and Physical Sciences, University of Exeter, Streatham Campus, North Park Road, Exeter, Devon EX4 4QF, United Kingdom;2. Faculty of Science and Technology, Federation University Australia, Mt Helen Campus, University Drive, Ballarat, Victoria 3350, Australia;3. School of Mechanical and Industrial Engineering, Mekelle University, Endayesus Campus, PO Box 3086, Mekelle, Tigray, Ethiopia;1. Department of Earth and Planetary Science, University of California, Berkeley, CA 94720, USA;2. Department of Earth and Planetary Science, Department of Astronomy, University of California, Berkeley, CA 94720, USA;1. Functional Omics and Bioprocess Development Laboratory, Biotechnology Program, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia;2. Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, 25200, Pahang, Malaysia;3. Halal Action Laboratory, Kolej Permata Insan, University Sains Islam Malaysia, Bandar Baru Nilai, 71800, Nilai, Negeri Sembilan, Malaysia;4. Institute for Food Technology and Biochemistry, Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080, Belgrade, Serbia;5. Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia;1. Departament d''Astronomia i Astrofísica, Universitat de València, Edifici d''Investigació Jeroni Munyoz, C/Dr. Moliner, 50, E-46100 Burjassot, València, Spain;2. Extreme Universe Laboratory, Skobeltsyn Institute of Nuclear Physics, Moscow State University by M.V. Lomonosov, Leninskie Gory, 119991 Moscow, Russian Federation;3. Max-Planck-Institut für Extraterrestrische Physik, Postfach 1312 D-85741 Garching, Bavaria, Germany |
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| Abstract: | Effects of Coix lacryma-jobi oil (CLO) addition on the mycelia growth and production of bioactive metabolites, such as triterpenoids, exopolysaccharide (EPS), and intracellular polysaccharide (IPS) in the submerged culture of Ganoderma lucidum were studied. The results showed that when a level of 2% CLO was added at the beginning of culture, the biomass, triterpenoids, EPS, and IPS productions reached a maximum of 10.71 g/L, 92.94 mg/L, 0.33 g/L, and 0.389 g/L, respectively, that were 3.34-fold, 2.76-fold, 2.2-fold, and 2.23-fold compared to that of control. Analysis of fermentation kinetics of G. lucidum suggested that glucose concentration in the culture of CLO-added group decreased more quickly as compared to the control group from day 2 to day 7 of fermentation process, while the triterpenoids and polysaccharides biosynthesis were promoted at the same culture period. However, the culture pH profile was not affected by the addition of CLO. There were no new components in the two types of polysaccharides obtained by the addition of CLO. Enzyme activities analysis indicated CLO or its fatty acids affected the synthesis level of phosphoglucose isomerase and α-phosphoglucomutase at different stage. |
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| Keywords: | Polysaccharide Biosynthesis Enzyme activity Fermentation Submerged culture |
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