Engineering Escherichia coli to convert acetic acid to free fatty acids |
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| Affiliation: | 1. Department of Energy, Environmental and Chemical Engineering, Washington University, St. Louis, MO 63130, USA;2. Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA;1. State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;2. Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai 200237, China;3. Key Laboratory of Bio-based Material Engineering of China National Light Industry Council, 130 Meilong Road, Shanghai 200237, China;1. Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China;2. The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, PR China;3. Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan;4. Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 70101, Taiwan;1. CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China;2. University of Chinese Academy of Sciences, Beijing, China;3. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China |
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| Abstract: | Fatty acids (FAs) are promising precursors of advanced biofuels. This study investigated conversion of acetic acid (HAc) to FAs by an engineered Escherichia coli strain. We combined established genetic engineering strategies including overexpression of acs and tesA genes, and knockout of fadE in E. coli BL21, resulting in the production of ~1 g/L FAs from acetic acid. The microbial conversion of HAc to FAs was achieved with ~20% of the theoretical yield. We cultured the engineered strain with HAc-rich liquid wastes, which yielded ~0.43 g/L FAs using waste streams from dilute acid hydrolysis of lignocellulosic biomass and ~0.17 g/L FAs using effluent from anaerobic-digested sewage sludge. 13C-isotopic experiments showed that the metabolism in our engineered strain had high carbon fluxes toward FAs synthesis and TCA cycle in a complex HAc medium. This proof-of-concept work demonstrates the possibility for coupling the waste treatment with the biosynthesis of advanced biofuel via genetically engineered microbial species. |
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| Keywords: | Anaerobic-digested Carbon flux Lignocellulosic biomass |
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