A comparative study: Immobilization of yeast cells and invertase in poly(ethyleneoxide) electrodes |
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| Affiliation: | 1. Department of Energy Systems, Soonchunhyang University, Asan 31538, Republic of Korea;2. Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul 03722, Republic of Korea;1. Department of Information Display Engineering, Hanyang University, Seoul, 04763, South Korea;2. Department of Electronics and Computer Engineering, Hanyang University, Seoul, 04763, South Korea;1. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China;2. State Key Laboratory of Precision Spectroscopy, Department of Physics, East China Normal University, Shanghai 200062, China;3. State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China |
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| Abstract: | Yeast cells (Saccharomyces cerevisiae) and invertase enzyme were immobilized in thiophene capped poly(ethyleneoxide)/polypyrrole (PEO-co-PPy) and 3-methylthienyl methacrylate-co-p-vinyl benzyloxy poly(ethyleneoxide)/polypyrrole (CP-co-PPy) matrices. Immobilization of the enzyme and yeast cells was performed via entrapment in conducting copolymers during electrochemical polymerization of pyrrole through the thiophene moiety of the polymers. Maximum reaction rates, Michaelis–Menten constants, optimum temperature and pH values, operational and storage stabilities of the enzyme and yeast cell electrodes were investigated. |
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