Metabolic engineering of Torulopsis glabrata for malate production |
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| Institution: | 1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China;2. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;3. Laboratory of Food Microbial-Manufacturing Engineering, Jiangnan University, Wuxi 214122, China;1. Université de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France;2. INRA, UMR792, Laboratoire d’Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France;3. CNRS, UMR5504, Laboratoire d’Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France;1. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China;2. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China;1. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;2. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China;3. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China;1. Vel Tech High Tech Dr. Rangarajan Dr. Sakunthala Engineering College, Chennai 600062, India;2. Department of Biotechnology, St. Joseph’s College of Engineering, Chennai 600119, India;3. Centre for Biotechnology, Anna University, Chennai 600025, India;1. Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;2. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;3. School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta 30332, USA |
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| Abstract: | The yeast Torulopsis glabrata CCTCC M202019, which is used for industrial pyruvate production, was chosen to explore the suitability of engineering this multi-vitamin auxotrophic yeast for increased malate production. Various metabolic engineering strategies were used to manipulate carbon flux from pyruvate to malate: (i) overexpression of pyruvate carboxylase and malate dehydrogenase; (ii) identification of the bottleneck in malate production by model iNX804; (iii) simultaneous overexpression of genes RoPYC, RoMDH and SpMAE1. Using these strategies, 8.5 g L–1 malate was accumulated in the engineered strain T.G-PMS, which was about 10-fold greater than that of the control strain T.G-26. The results presented here suggest that T. glabrata CCTCC M202019 is a promising candidate for industrial malate production. |
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| Keywords: | Malate Metabolic engineering Genome-scale metabolic model |
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