Optimization and kinetic study on the synthesis of palm oil ester using Lipozyme TL IM |
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| Institution: | 1. Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;2. Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Serdang, Selangor, Malaysia;3. Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;1. Institute of Applied Chemistry, Brandenburg University of Technology Cottbus-Senftenberg, Platz der Deutschen Einheit 1, D-03046 Cottbus, Germany;2. Chair of Chemical Reaction Engineering, Brandenburg University of Technology Cottbus-Senftenberg, Burger Chaussee 2, D-03044 Cottbus, Germany;1. Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain;2. Crystallographic Studies Laboratory, Andalusian Institute of Earth Sciences, CSIC, Avd. Las Palmeras, n°4, 18100 Armilla, Granada, Spain;3. Department of Microbiology, University of Córdoba, Campus de Rabanales, Ed. Severo Ochoa, 14014 Córdoba, Spain;4. Seneca Green Catalyst S.A., Edif. Centauro, Parque Científico Tecnológico de Córdoba, Rabanales 21, 14014 Córdoba, Spain;1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, PR China;2. School of Food Science and Engineering, Guangdong Research Center of Lipid Science and Applied Engineering Technology, South China University of Technology, Guangzhou, 510641, PR China;1. Department of Pharmaceutical Engineering, College of Chemical Engineering, Qingdao University of Science and Technology, Mail box 70, 53 Zhengzhou Road, Qingdao, 266042, China;2. State Key Laboratory Base for Eco-Chemical Engineering in College of Chemical Engineering, Qingdao University of Science and Technology, Mail box 70, 53 Zhengzhou Road, Qingdao, 266042, China;1. Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China;2. Amoy – BUCT Industrial Bio-Technovation Institute, Amoy 361022, PR China;3. State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China |
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| Abstract: | Enzymatic synthesis of palm oil esters (POE) was carried out via alcoholysis of palm oil (PO) and oleyl alcohol (OA) catalyzed by Lipozyme TL IM. The optimum reaction conditions were: temperature: 60 °C; enzyme load: 24.7 wt%; substrate ratio: 1:3 (PO/OA), impeller speed: 275 rpm and reaction time: 3 h. At the optimum condition, the conversion of POE was 79.54%. Reusability study showed that Lipozyme TL IM could be used for 5 cycles with conversion above 50%. The alcoholysis reaction kinetic follows the Ping-Pong Bi-Bi mechanism characterized by the Vmax, Km(PO), and Km(OA) values of 32.7 mmol/min, 0.3147 mmol/ml and 0.9483 mmol/ml, respectively. The relationship between initial reaction rate and temperature was also established based on the Arrhenius law. |
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