Improved synthesis of 1,3-diolein by Novozym 435-mediated esterification of monoolein with oleic acid |
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| Affiliation: | 1. Academy of State Administration of Grain, Beijing 100037, PR China;2. Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China;1. College of Life Science, Fujian Normal University, Fuzhou 350117, China;2. Engineering Research Center of Industrial Microbiology of Ministry of Education, Fujian Normal University, Fuzhou 350117, China;3. Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark;1. Bioprocess Engineering and Biotechnology, Federal University of Tocantins – UFT, Rua Badejós, Chácaras 69/72, Zona Rural, Gurupi, Tocantins, 77402-970, Brazil;2. Biochemistry and Microbiology Department, Bioscience Institute, São Paulo State University, IB/UNESP, Av. 24-A, 1515, Bela Vista, Rio Claro, São Paulo, 13506-900, Brazil;3. Environmental Studies Center, São Paulo State University, CEA/UNESP, Av. 24-A, 1515, Bela Vista, Rio Claro, São Paulo, 13506-900, Brazil;1. Biotechnology, Bioprocess and Biocatalysis Group, Institute of Food Science and Technology, Federal University of Rio Grande do Sul State, Av. Bento Gonçalves, 9500, PO Box 15090, ZC 91501-970 Porto Alegre, RS, Brazil;2. Department of Biocatalysis, ICP-CSIC, Campus UAM-CSIC, Cantoblanco, ZC 28049 Madrid, Spain;1. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China;2. College of Food Sciences, Southwest University, Chongqing 400715, China;3. Guangdong VTR Bio-Tech Co., Ltd., Zhuhai 519060, China;4. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China |
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| Abstract: | We successfully developed an efficient and promising bioprocess for 1,3-diolein synthesis by performing Novozym 435-mediated esterification of oleic acid with monoolein in this work. Under the optimized conditions (60 °C, molar ratio of oleic acid to monoolein 1.2:1), a 1,3-diolein yield of 93.7% could be achieved, and the yield of 1,2-diolien was low (2.6%). The high yield of 1,3-diolein and the optimum reaction time were improved remarkably compared with the results of our previous study, which involved the enzymatic esterification of oleic acid with glycerol. An additional advantage of the new process is the fact that 90% original activity of the enzyme was maintained after being used for 100 reactions. The present work could be seen as a useful enzyme-catalyzed process for the industrial production of 1,3-diacylglycerol. |
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