Engineering cyanobacteria for photosynthetic production of 3-hydroxybutyrate directly from CO2 |
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| Institution: | 1. Center for Biosignatures Discovery Automation, The Biodesign Institute, Arizona State University, Tempe, AZ 85287-6501, USA;2. Biological Design Graduate Program, Arizona State University, Tempe, AZ, USA;3. Chemical Engineering Program, Ira A. Fulton Schools of Engineering, Arizona State University, Tempe, AZ 85287-6106, USA;1. CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China;2. Department of Cellular Biology, University of Science and Technology of China, Hefei, China;1. Laboratory of Cyanobacterial Biotechnology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand;2. Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Thanyaburi, Pathumthani 12110, Thailand;3. Microbial Chemistry, Department of Chemistry – Ångström, Uppsala University, Box 523, SE-75120 Uppsala, Sweden;1. Biosciences Center, National Renewable Energy Laboratory, Golden, CO, USA;2. Institute of Plant Biology, National Taiwan University, Taipei, Taiwan;3. Institute of Pharmacology, Kaohsiung Medical University, Kaohsiung, Taiwan;4. Department of Genome Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;1. Institute of Chemical, Environmental and Bioscience Engineering, Research Area Biochemical Engineering, Technische Universität Wien, 1060 Vienna, Austria;2. Institute of Chemical, Environmental and Bioscience Engineering, Research Group for Microbiology and Applied Genomics, Technische Universität Wien, 1060 Vienna, Austria;3. Lackner Ventures & Consulting GmbH, Hofherr Schrantz Gasse 2, 1210 Vienna, Austria;4. University of Applied Sciences FH Technikum Wien, 1200 Vienna, Austria;5. CD Laboratory on Mechanistic and Physiological Methods for Improved Bioprocesses, Technische Universität Wien, 1060 Vienna, Austria;1. Laboratory for Bioinformatics, Graduate School of Systems Biosciences, Kyushu University, 804 Westwing, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan;2. Laboratory of Plant Physiology and Metabolism, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai, Tsukuba 305-8572, Japan |
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| Abstract: | (S)- and (R)-3-hydroxybutyrate (3HB) are precursors to synthesize the biodegradable plastics polyhydroxyalkanoates (PHAs) and many fine chemicals. To date, however, their production has been restricted to petroleum-based chemical industry and sugar-based microbial fermentation, limiting its sustainability and economical feasibility. With the ability to fix CO2 photosynthetically, cyanobacteria have attracted increasing interest as a biosynthesis platform to produce fuels and chemicals from alternative renewable resources. To this end, synthesis metabolic pathways have been constructed and optimized in cyanobacterium Synechocystis sp. PCC 6803 to photosynthetically produce (S)- and (R)-3HB directly from CO2. Both types of 3HB molecules were produced and readily secreted from Synechocystis cells without over-expression of transporters. Additional inactivation of the competing pathway by deleting slr1829 and slr1830 (encoding PHB polymerase) from the Synechocystis genome further promoted the 3HB production. Up to 533.4 mg/L 3HB has been produced after photosynthetic cultivation of the engineered cyanobacterium Synechocystis TABd for 21 days. Further analysis indicated that the phosphate consumption during the photoautrophic growth and the concomitant elevated acetyl-CoA pool acted as a key driving force for 3HB biosynthesis in Synechocystis. For the first time, the study has demonstrated the feasibility of photosynthetic production of (S)- and (R)-3HB directly from sunlight and CO2. |
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