A novel and environment-friendly bioprocess of 1,3-propanediol fermentation integrated with aqueous two-phase extraction by ethanol/sodium carbonate system |
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| Affiliation: | 1. School of Chemistry & Chemical Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou 510640 PR China;2. Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164, USA;1. School of Biological and Chemical Engineering, Jiangsu Food and Pharmaceutical Science College, Huai’an, Jiangsu 223003, China;2. Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian 361005, China;3. The Key Laboratory for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, Fujian 361005, China;4. National Engineering Laboratory for Green Chemical Productions of Alcohols-Ethers-Ester, Xiamen University, Xiamen, Fujian 361005, China;5. The Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, Fujian 361005, China;1. Departamento de Engenharia Química, Universidade de São Paulo, São Paulo, SP, Brazil;2. Centro de Capacitação e Pesquisa em Meio Ambiente (CEPEMA), São Paulo, SP, Brazil;1. School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, PR China;2. Chemical Control Technology Key Laboratory of Liaoning Province, Institute of Information and Engineering, Shenyang University of Chemical and Technology, Shenyang 110142, PR China;3. College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, PR China |
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| Abstract: | An integrated fermentation–separation process for the production of 1,3-propanediol (1,3-PD) was investigated. Aqueous two-phase system (ATPS) not only recovered 97.9% of 1,3-PD, but simultaneously also removed 99.1% cells, 81.9% proteins, 75.5% organic acids, and 78.7% water. Furthermore, after extraction the bottom phase of ATPS was used to adjust the pH of the culture during fermentation, leading to 16% and 126% increases in the concentrations of 1,3-PD and lactic acid, and dramatic decreases in the concentration of acetic acid and formic acid. The total mass conversion yield of three main products (1,3-PD, 2,3-butanediol, and lactic acid) from glycerol reached 81.6%. The salt-enriched phase could also be used to absorb carbon dioxide (CO2), resulting in 94% recovery for carbonate. Finally, process simulation using the program PRO/II showed the use of ATPS reduced 75.1% of the energy expenditure and 89.0% of CO2 emissions. |
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| Keywords: | 1,3-Propanediol Fermentation Aqueous two-phase extraction |
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