Kinetic study on enzymatic esterification of tuna fish oil fatty acids with butanol |
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| Institution: | 1. Department of Chemical Engineering, Malaviya National Institute of Technology, Jaipur 302017, India;2. Catalysis and Chemical Engineering Laboratories, Chemical and Biological Engineering Department, University of Saskatchewan, Saskatoon, SK, Canada S7N 5A9;1. Growth, Exercise, NUtrition and Development (GENUD) Research Group, University of Zaragoza, Food and Agriculture Institute of Aragón (IA2), Health Research Institute of Aragón (ISS Aragón), Zaragoza, Spain;2. Department of Biochemistry and Molecular Biology II, Institute of Nutrition and Food Technology, University of Granada, Biomedical Research Institute ibs, Granada, Spain;3. CIBER Obesity and Nutrition Physiopathology (CIBEROBN), Madrid, Spain;4. Paediatric Research and Metabolism Unit, Reina Sofía University Hospital, Maimonides Institute for Biomedical Research of Córdoba (IMBIC), Córdoba, Spain;5. Unit of Investigation in Nutrition, Growth and Human Development of Galicia, Paediatric Department, Health Research Institute of Santiago (IDIS), University of Santiago de Compostela, Galicia, Spain;6. Paediatric Department, Lozano Blesa University Hospital, University of Zaragoza, Food and Agriculture Institute of Aragón (IA2), Health Research Institute of Aragón (ISS Aragón), Zaragoza, Spain;1. Faculty of Food Science and Technology, University Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia;2. Institute of Technology, Middle Technical University, 29008 Alzafaranya, Baghdad, Iraq;3. Halal Products Research Institute, University Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia;1. Department of Food Science and Nutrition, Zhejiang University, Hangzhou, China;2. Department of Epidemiology, Capital Institute of Pediatrics, Beijing, China;3. Department of Nutrition, Chinese PLA General Hospital, Beijing, China;4. Medical Research Council Epidemiology Unit, University of Cambridge, Cambridge, UK;5. Institute of Nutrition & Health, Qingdao University, Qingdao, China;1. Department of Chemical Engineering, Engineering School of Lorena, University of São Paulo, 12602-810, Lorena, SP, Brazil;2. Combustion and Propulsion Associated Laboratory, National Institute for Space Research, 12630-000, Cachoeira Paulista, SP, Brazil;1. Department of Chemical Engineering and Food Engineering, UFSC, Florianópolis, SC, Brazil;2. Department of Biochemistry, Biological Sciences Center, UFSC, Florianópolis, SC, Brazil |
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| Abstract: | Docosahexaenoic acid (DHA) is an important polyunsatured fatty acid (PUFA) which can be purified from tuna fish oil fatty acids by selective enzymatic esterification. The present paper investigates the kinetic study for selective esterification of tuna fish oil fatty acids with butanol catalyzed by Rhizopus oryzae lipase (ROL) in biphasic solvent system. Under the most suitable reaction conditions, 76.2% esterification was achieved in 24 h. Different kinetic models for esterification given by Segel 1], Oliveira et al. 2], Gogoi et al. 3], and Kraai et al. 4] were tested for fitting the esterification data and the model given by Oliveira et al. 2] was found to be most suitable. The model given by Prazeres et al. 5] for hydrolysis was also tested for esterification and the model with second order product inhibition was found to provide better match between the predicted and experimental values than that of model by Oliveira et al. 2]. The kinetic model was fitted using MATLAB® to determine the best kinetic parameters. The average value of kinetic constants using the model given by Prazeres et al. were estimated as Km = 23.6 μmoles FFA/ml, Ki1 = 4.6 × 10−5 μmoles FFA/mg enzyme h, Ki2 = 0.0062 μmoles FFA/mg enzyme h and K2 = 149.5 μmoles FFA/mg enzyme h. |
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| Keywords: | Tuna fish oil Selective esterification Biphasic solvent system |
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