Characterization of the mechanism of action of the genetically modified Cry1AbMod toxin that is active against Cry1Ab-resistant insects |
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Authors: | Carlos Muñóz-Garay Liliana Pardo-López Ivan Arenas Rosana Sánchez-López Andreas Holzenburg Christos G Savva Alejandra Bravo |
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Institution: | a Instituto de Biotecnología, Universidad Nacional Autónoma de México. Apdo. postal 510-3, Cuernavaca 62250, Morelos, Mexico b Department of Biology and Department of Biochemistry and Biophysics, Texas A&M University. College Station, Texas 77843-2257, USA c Microscopy and Imaging Center, Texas A&M University. College Station, Texas 77843-2257, USA |
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Abstract: | Bacillus thuringiensis Cry toxins are used in the control of insect pests. They are pore-forming toxins with a complex mechanism that involves the sequential interaction with receptors. They are produced as protoxins, which are activated by midgut proteases. Activated toxin binds to cadherin receptor, inducing an extra cleavage including helix α-1, facilitating the formation of a pre-pore oligomer. The toxin oligomer binds to secondary receptors such as aminopeptidase and inserts into lipid rafts forming pores and causing larval death. The primary threat to efficacy of Bt-toxins is the evolution of insect resistance. Engineered Cry1AMod toxins, devoid of helix α-1, could be used for the control of resistance in lepidopterans by bypassing the altered cadherin receptor, killing resistant insects affected in this receptor. Here we analyzed the mechanism of action of Cry1AbMod. We found that alkaline pH and the presence of membrane lipids facilitates the oligomerization of Cry1AbMod. In addition, tryptophan fluorescence emission spectra, ELISA binding to pure aminopeptidase receptor, calcein release assay and analysis of ionic-conductance in planar lipid bilayers, indicated that the secondary steps in mode of action that take place after interaction with cadherin receptor such as oligomerization, receptor binding and pore formation are similar in the Cry1AbMod and in the wild type Cry1Ab. Finally, the membrane-associated structure of Cry1AbMod oligomer was analyzed by electron crystallography showing that it forms a complex with a trimeric organization. |
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Keywords: | Bt Bacillus thuringiensis Cry crystal proteins SUV small unilamellar vesicles PC phosphatidylcholine AFU arbitrary fluorescence units PMSF Phenylmethanesulphonyl fluoride PVDF polyvinylidene difluoride HRP horseradish peroxidase ELISA enzyme linked immunosorbent assay 2D two-dimensional OTG d-1-thioglucopyranoside" target="_blank">octyl-β-d-1-thioglucopyranoside |
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