Biophysical characterization of the fusogenic region of HCV envelope glycoprotein E1 |
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Authors: | Ana J. Pé rez-Berná ,Peter Laggner |
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Affiliation: | a Instituto de Biología Molecular y Celular, Universidad “Miguel Hernández”, E-03202 Alicante, Spain b Institute of Biophysics and Nanosystems Research, Austrian Academy of Sciences, Graz, Austria |
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Abstract: | We have studied the binding and interaction of the peptide E1FP with various model membranes. E1FP is derived from the amino acid segment 274-291 of the hepatitis C virus envelope glycoprotein E1, which was previously proposed to host the peptide responsible for fusion to target membranes. In the present study we addressed the changes which take place upon E1FP binding in both the peptide and the phospholipid bilayer, respectively, through a series of complementary experiments. We show that peptide E1FP binds to and interacts with phospholipid model membranes, modulates the polymorphic phase behavior of membrane phospholipids, is localized in a shallow position in the membrane and interacts preferentially with cholesterol. The capability of modifying the biophysical properties of model membranes supports its role in HCV-mediated membrane fusion and suggests that the mechanism of membrane fusion elicited by class I and II fusion proteins might be similar. |
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Keywords: | 16NS, 16-Doxyl-stearic acid 5NS, 5-Doxyl-stearic acid BPS, Bovine brain L-α-phosphatidylserine CF, 5-Carboxyfluorescein CHOL, Cholesterol DEPE, 1,2-Dielaidoyl-sn-glycero-3-phosphatidylethanolamine DHE, Dehydroergosterol di-8-ANEPPS, 4-(2-(6-(Dioctylamino)-2-naphthalenyl)-(ethenyl)-1-(3-sulfopropyl)-pyridinium inner salt EPA, Egg L-α-phosphatidic acid EPC, Egg L-α-phosphatidylcholine EPG, Egg L-α-phosphatidylglycerol ESM, Egg sphingomyelin FPE, Fluorescein-phosphatidylethanolamine HCV, Hepatitis C virus LV, Liver lipid extract LUV, Large unilamellar vesicles MLV, Multilamellar vesicles NBD-PE, N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-phosphoethanolamine N-RhB-PE, Lissamine&trade rhodamine B 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine POPC, 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine SAXD, Small angle X-ray diffraction Tm, Temperature of the gel-to-liquid crystalline phase transition |
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