Cloning of a cuticle-degrading protease from the entomopathogenic fungus, Beauveria bassiana |
| |
Authors: | Lokesh Joshi Raymond J. St. Leger Michael J. Bidochka |
| |
Affiliation: | Boyce Thompson Institute, Tower Road, Ithaca, NY 14853-1801, USA |
| |
Abstract: | Abstract A Beauveria bassiana extracellular subtilisin-like serine endoprotease is a potential virulence factor by virtue of its activity against insect cuticles. A cDNA clone of the protease was isolated from mycelia of B. bassiana grown on cuticle/chitin cultures. The amino acid sequence of this gene was compared to that of Metarhizium anisopliae Pr1, the only pathogenicity determinant so far described from an entomopathogenic fungus, and proteinase K, isolated from Tritirachium album , a saprophytic fungus. The cDNA sequence revealed that B. bassiana Prl is synthesized as a large precursor ( M r 37 460) containing a signal peptide, a propeptide and the mature protein predicted to have an M r of 26 832. |
| |
Keywords: | Beauveria bassiana Protease gene DNA sequence Cuticle degradation Beauveria bassiana Pr1 |
本文献已被 ScienceDirect 等数据库收录! |
|