Sodium azide-induced mutagenesis in Saccharomyces cerevisiae. |
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Authors: | L Silhánková V Smiovská J Velemínsky |
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Institution: | 1. Department of Biochemistry and Microbiology, Institute of Chemical Technology, 16628 Praha 6, Suchbátarova 5, Czechoslovakia;1. Institute of Experimental Botany, Czechoslovak Academy of Sciences, 160 00 Praha 6, Flemingovo 2 Czechoslovakia |
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Abstract: | Sodium azide (0.5--2.0 X 10(-5) M), applied for 24 h on cells growing in complete medium, increased up to 26 times the frequency of reversions and locus-specific suppressor mutations of allele ilv1-92 in diploid strain D7 of Saccharomyces cerevisiae. Similarly, it enhanced the frequency of reversions and/or mitotic gene conversions of alleles trp5-12/trp5-27 up to 19 times. Reconstruction experiments showed that the increase of mutations in complete medium was not due to a selection of prototrophic types under growth conditions and, therefore, that sodium azide acts as a weak mutagen in S. cerevisiae under growth conditions at a low pH. No mutagenic or convertogenic effect was observed when azide was applied to resting cells in buffer at pH 4.2. |
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