Hydrogen-using bacteria in a methanogenic acetate enrichment culture |
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Authors: | DB Archer |
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Institution: | ARC Food Research Institute, Colney Lane, Norwich NR4 7UA |
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Abstract: | A rcher , D.B. 1984. Hydrogen-using bacteria in a methanogenic acetate enrichment culture. Journal of Applied Bacteriology 56 , 125–129. In a study of the anaerobic utilization of acetate, an enrichment culture of sewage sludge organisms was initiated with calcium acetate as the sole carbon and energy source. A mixed bacterial population became established from which 14 anaerobic species were isolated. Two of the isolates were methanogenic bacteria but only one of these, Methanosarcina barkeri , utilised acetate as an energy source in axenic culture. The other methanogenic isolate, a Methanobacterium sp., utilised H2/CO2 but not acetate. A third methanogen, which was morphologically identical to Methanothrix soehngenii , was detected in the enrichment but was not obtained in monoculture. 2-Bromoethanesulphonate, a specific inhibitor of methanogenesis. completely inhibited the enrichment at a concentration of 10 μmol/1. Addition of H2 formate or methanol to the enrichment did not affect the rate of methanogenesis. An H2-utilizing Desulfovibrio sp. was also isolated from the enrichment. |
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