Investigation on the interaction of nanoAg with Cu–Zn SOD

Silver nanoparticles (nanoAg) are used more and more widely, particularly because of their antimicrobial properties. The effect of exposure to nanoAg on the structure of superoxide dismutase (SOD) was thoroughly investigated using fluorescence measurements, synchronous fluorescence spectroscopy, steady‐state and time‐resolved fluorescence quenching measurements, UV/Vis absorption spectroscopy, resonance light scattering (RLS), circular dichroism (CD), isothermal titration calorimetry (ITC) and high‐resolution transmission electron microscopy (HRTEM). Through van der Waal's force, nanoAg interacted with Cu–Zn SOD and influenced the active site by inducing structural changes, which influenced the function of SOD. The fluorescence studies show that both static and dynamic quenching processes occur. This paper provides reference data for toxicological studies of nanoAg, which are important in the future development of nanotechnology. Copyright © 2015 John Wiley & Sons, Ltd.