The preparation of ethylenediamine‐modified fluorescent carbon dots and their use in imaging of cells |
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| Authors: | Wei Dong Siqi Zhou Yan Dong Jingwen Wang Xin Ge Lili Sui |
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| Affiliation: | 1. Department of Chemistry, Shenyang Medical College, Shenyang, People's Republic of China;2. ICU, Fengtian Hospital Affiliated to Shenyang Medical College, Shenyang, People's Republic of China;3. Experiment Center of Traditional Chinese Medicine Department, Shenyang Pharmaceutical University, People's Republic of China |
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| Abstract: | In this work, fluorescent carbon dots (CDs) were synthesized using a hydrothermal method with glucose as the carbon source and were surface‐modified with ethylenediamine. The properties of as‐prepared CDs were analyzed by transmission electron microscopy (TEM), Fourier transform infrared (FTIR), ultraviolet–visible light (UV/vis) absorption and fluorescent spectra. Furthermore, CDs conjugated with mouse anti‐(human carcinoembryonic antigen) (CEA) monoclonal antibody were successful employed in the biolabeling and fluorescent imaging of human gastric carcinoma cells. In addition, the cytotoxicity of CDs was also tested using human gastric carcinoma cells. There was no apparent cytotoxicity on human gastric carcinoma cells. These results suggest the potential application of the as‐prepared CDs in bioimaging and related fields. Copyright © 2015 John Wiley & Sons, Ltd. |
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| Keywords: | carbon dots ethylenediamine cell imaging cytotoxicity |
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