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Near-infrared fluorescence detection of ATP-biotin-mediated phosphoprotein labeling |
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| Authors: | Xiaofei Gao Amy Schutz-Geschwender Philip R Hardwidge |
| Institution: | (1) Center for Infectious Disease Research and Vaccinology, South Dakota State University, Box 2175, 1119 North Campus Drive, Brookings, SD 57007, USA;(2) LI-COR Biosciences, Lincoln, NE 68504, USA |
| Abstract: | Detection of phosphoproteins plays an important role in understanding protein function in cellular signalling pathways. Improved methods for identification and quantification of phosphoproteins are research priorities. Near-infrared (NIR) fluorescence detection of a γ-modified ATP-biotin analog was used to detect protein phosphorylation, using both model kinase substrates and mammalian cell lysates. NIR signal intensity was dependent on substrate and ATP-biotin concentrations. |
| Keywords: | γ -modified ATP-biotin Near-infrared (NIR) fluorescence Phosphoprotein Protein kinase |
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