Arabidopsis ENOR3 regulates RNAi-mediated antiviral defense |
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| Authors: | Hua Gao Mai Yang Haitao Yang Yue Qin Biyun Zhu Gang Xu Chengyuan Xie Dewei Wu Xiaolin Zhang Wanxiang Li Jianbin Yan Susheng Song Tiancong Qi Shou-Wei Ding Daoxin Xie |
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| Affiliation: | 1. Tsinghua-Peking Joint Center for Life Sciences, and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China;2. Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521, USA;3. Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, College of Life Sciences, Capital Normal University, Beijing 100048, China |
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| Abstract: | Viruses can infect host plants to cause severe diseases and substantial agricultural loss, while plants have evolved RNA interference (RNAi) strategy to defend against viral infection. Despite enormous efforts, only a few host proteins in RNAi pathway were shown to mediate antiviral defense, including RNA-dependent RNA polymerase 1 (RDR1), RDR6, DICER-LIKE 2 (DCL2) and DCL4. In this study, we carried out a genetic screen for antiviral factors of RNAi pathway in Arabidopsis rdr6 background via inoculation with a 2b-deficient Cucumber Mosaic Virus (CMV-Δ2b). We identified a mutant susceptible to CMV-Δ2b, referred to as enhancer of rdr6 (enor) 3-1 rdr6, and found that ENOR3 encodes a functionally unknown protein with high homology to the mammalian Non Imprinted in Prader-Willi/Angelman (NIPA) magnesium transporters. ENOR3 inhibits accumulation of CMV-Δ2b and acts additively with RDR1, RDR6, DCL2 and DCL4 in antiviral defense. These results uncover that ENOR3 is a key component in antiviral RNAi pathway, and provide new insights into antiviral immunity. |
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| Keywords: | Antiviral defense CMV-Δ2b DCL2/4 ENOR3 RDR1/6 RNAi |
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