Down-regulation of circulating microRNA let-7a in Egyptian smokers |
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| Authors: | Sanaa A. Rizk Fateheya M. Metwally Asmaa M. Elfiky Asmaa A. Mahmoud Nadia A. Badawi Nevin E. Sharaf Mahmoud M. Elhefnawi |
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| Affiliation: | 1. Occupational and Environmental Medicine Department, Environmental Research Division, National Research Centre, Cairo, Egypt;2. Department of Zoology, Faculty of Science, Ain Shams University, Cairo, Egypt;3. Informatics and Systems Department, Engineering Research division, National Research Centre, Cairo, Egypt |
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| Abstract: | Altered miRNAs were associated with cigarette smoking. The study aimed to examine the gene expression level of plasma let-7a among healthy smokers and compared it with the non-smokers. Forty subjects were recruited for the present study and classified into 21 smokers and 19 non-smokers, age, and sex were matched. The software that used to design functional primers was MIRprimer. Quantitative real-time PCR was employed to compare the relative expression of plasma let-7a. Results showed that the level of let-7a was down-regulated in smokers to 0.34fold (p?=?0.006) that of the non-smokers. Plasma let-7a showed an area under curve (AUC) of 0.749 with sensitivity 43% and specificity 100%. In conclusion, plasma let-7a was significantly down-regulated in the smokers, and it might be considered a candidate biomarker to discriminate between smokers and non-smokers. |
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| Keywords: | Plasma let7-a Biomarker Cigarette smoking Software MIRprimer |
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