| 伤寒Vi多糖疫苗大规模生产中的工艺改进 |
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| 引用本文： | 钟泽荣 沈吉友. 伤寒Vi多糖疫苗大规模生产中的工艺改进[J]. 微生物学免疫学进展, 1997, 25(1): 20-23 |
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| 作者姓名： | 钟泽荣 沈吉友 |
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| 作者单位： | 卫生部成都生物制品研究所 |
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| 摘 要： | 对伤寒Ｖｉ多糖疫苗大规模生产进行了三项工艺改进：１．将液体菌种由静止期接种大罐改为对数生长期接种大罐，大罐培养时间可缩短３－４小时。２．２％（Ｖ／Ｖ）甲醛溶液杀菌时间由５℃６小时改为２０－２５℃２小时，能保证杀菌完全。３．用冷酚精制Ｖｉ多糖由常规法改为乳化法，可减少精制次数及冷酚用量，提高多糖收率
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| 关 键 词： | Vi多糖 对数期 静止期 乳化 |
Improved Manufacturing Technique of Vi Polysaccharide Typhoid Vaccine (VPTV) |
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| Zhong Zherong,Shen Jiyou,Xiong Huiling,et al. Improved Manufacturing Technique of Vi Polysaccharide Typhoid Vaccine (VPTV)[J]. Progress In Microbiology and Immunology, 1997, 25(1): 20-23 |
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| Authors: | Zhong Zherong Shen Jiyou Xiong Huiling et al |
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| Abstract: | Three methods of technological improvements in process used for the large scale VPTV as were described follows:1.The fermentation time is shortened by 3-4hrs,using inoculated seed stock suspension in exponential phase instead of in stationary phase;2.Bacteria killed time is shortened to 2hrs with 2% formaline at 20-25℃ instead of 6hrs at 5℃;3。To purify Vi polysaccharide by cold phenol extraction with the emulsified solution instead of by a conuentional method without emulsification can reduce purification times and amounts of phenol consumption and increase the yield of products. |
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| Keywords: | Vi Polysaccharide exponential phase Stationary phase Emulsification |
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