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| 产琥珀酸工程菌株的发酵工艺条件优化 | |
| 引用本文： | 尹强,康振,钟盛华,祁庆生.产琥珀酸工程菌株的发酵工艺条件优化[J].生物加工过程,2010,8(4):1-5. |
| 作者姓名： | 尹强 康振 钟盛华 祁庆生 |
| 作者单位： | 1. 山东大学,微生物技术国家重点实验室,济南,250100;江西农业大学,理学院,南昌,330045 2. 山东大学,微生物技术国家重点实验室,济南,250100 3. 江西农业大学,理学院,南昌,330045 |
| 基金项目： | 国家重点基础研究发展计划(973计划)资助项目，国家高技术研究发展计划(863计划)资助项目 |
| 摘 要： | 对实验室构建的产琥珀酸大肠杆菌工程菌株(E.coliQZ1111)进行发酵工艺条件研究。以AM1低盐培养基为基础,研究不同C、N源及其质量浓度,培养基初始pH和发酵温度等因素对琥珀酸的影响,并在5L发酵罐中进行了补料-分批发酵实验。优化后的发酵条件为葡萄糖20g/L,玉米浆10g/L,pH6.4,发酵温度37℃。在5L发酵罐中培养,琥珀酸产量达到47.9g/L。 |
| 关 键 词： | 琥珀酸 发酵 大肠杆菌 |
Optimization of fermentation production of succinate engineered by Escherichia coli |
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| YIN Qiang,KANG Zhen,ZHONG Sheng-hua,QI Qing-sheng.Optimization of fermentation production of succinate engineered by Escherichia coli[J].Chinese Journal of Bioprocess Engineering,2010,8(4):1-5. | |
| Authors: | YIN Qiang KANG Zhen ZHONG Sheng-hua QI Qing-sheng |
| Institution: | YIN Qiang 1,2,KANG Zhen 1,ZHONG Sheng-hua 2,QI Qing-sheng 1(1.State Key Laboratory of Microbial Techndogy,Shandong University,Ji'nan 250100,China,2.College of Sciences,Jiangxi Agricultural University,Nanchang 330045,China) |
| Abstract: | Aerobic succinate production was conducted with an engineered Escherichia coli QZ1111.Influences of carbon sources,nitrogen sources,initial pH,and temperature on the succinate production were investigated.The optimized conditions were glucose 20 g/L,corn steep liquor 10 g/L,pH 6.4,and temperature 37 ℃.Fed-batch fermentation in a 5 L fermenter was tested.The succinate concentration reached 47.9 g/L in the 5 L fermenter. |
| Keywords: | succinate fermentation Escherichia coli |
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