Construction of an Escherichia coli K-12 strain deleted for manganese and iron superoxide dismutase genes and its use in cloning the iron superoxide dismutase gene of Legionella pneumophila. |
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| Authors: | Howard M. Steinman |
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| Affiliation: | (1) Albert Einstein College of Medicine, Department of Biochemistry, 1300 Morris Park Avenue, 10461 Bronx, NY, USA |
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| Abstract: | Summary An Escherichia coli K-12 strain deleted for sodA and sodB (manganese and iron superoxide dismulases) was constructed and characterized by Southern blotting, enzyme assays, and physiological analyses. The sod deletion strain was used to clone the iron superoxide dismutase gene of Legionella pneumophila by complementation to paraquat resistance. |
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| Keywords: | Superoxide dismutase Deletion mutants Escherichia coli Legionella pneumophila |
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