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Evidence for a geranyl-diphosphate synthase located within the plastids of Vitis vinifera L. cultivated in vitro |
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| Authors: | E. Soler G. Feron M. Clastre R. Dargent M. Gleizes C. Ambid |
| Affiliation: | (1) Laboratoire de Biotechnologie et Amélioration des Plantes, Ecole Nationale Supérieure Agronomique, 145 avenue de Muret, F-31076 Toulouse Cédex, France;(2) Laboratoire de Cryptogamie, Université Paul Sabatier, 118 route de Narbonne, F-31062 Toulouse Cédex, France;(3) Laboratoire de Physiologie Cellulaire Végétale, Université de Bordeaux-I, avenue des facultés, F-33405 Talence Cédex, France |
| Abstract: | Intact plastids from cell suspensions of Vitis vinifera L. cv. Muscat de Frontignan, free of detectable contamination by other particles as judged by the distribution of organelle-specific marker enzymes and by electron microscopy, exhibit geranyl-diphosphate synthase activity (EC 2.5.1.1). This synthase activity remains stable after tryptic digestion of unlysed organelles and is enhanced by plastid disruption. We conclude that the enzyme is located within the organelle. The possibility of an isopentenyl diphosphate/dimethylallyl diphosphate translocating system which would play a major role in the regulation of monoterpene metabolism is discussed. |
| Keywords: | Cell culture (enzyme localization) Geranyl diphosphate synthase Monoterpene Plastid (monoterpene synthesis) Vitis |
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