Secretion of bovine alpha-lactalbumin into the milk of transgenic rats. |
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| Authors: | S Hochi T Ninomiya M Waga-Homma J Sagara A Yuki |
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| Affiliation: | Research Institute of Life Science, Snow Brand Milk Products, Co., Ltd., Tochigi, Japan. |
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| Abstract: | Bovine alpha-lactalbumin (b alpha LA) gene prepared by polymerase chain reaction was introduced into rat genomes by microinjection. Out of 17 transgenic rat lines, 11 secreted b alpha LA into their milk at concentrations higher than 0.2 micrograms/ml. Of these, three lines secreted b alpha LA at concentrations higher than those in bovine milk. The highest concentration of b alpha LA secreted into rat milk was 2,400 micrograms/ml. |
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| Keywords: | Milk proteins Mammary gland PCR Microinjection ELISA Expression |
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