Comparative large-scale propagation of retroviruses from old world (mason-pfizer monkey virus) and new world (squirrel monkey virus) primates |
| |
Authors: | Charles V Benton Howard M Hodge Donald L Fine |
| |
Institution: | (1) Viral Oncology Program, NCI Frederick Cancer Research Center, 21701 Frederick, Maryland |
| |
Abstract: | Summary A cell culture method is described for the large-scale (50 to 150 1) production of Mason-Pfizer monkey virus and squirrel
monkey virus, two primate retroviruses. Virus production was achieved with suspension cultures of chronically infected A204
human rhabdomyosarcoma cells harvested and clarified in the logarithmic stages of cell culture growth. Methods for the subsequent
purification and concentration of virus material utilizing zonal centrifugation also are described. Applications of these
methodologies resulted in products that afforded biochemical comparisons of these agents in a manner such that host cell-derived
variations were minimized. These data indicated that high levels of production and efficient recovery and purification of
virus material were achieved.
This work was supported by Contract NO1-CO-25423 with the National Cancer Institute, National Institutes of Health. |
| |
Keywords: | primate retroviruses suspension culture large-scale production |
本文献已被 SpringerLink 等数据库收录! |