中华按蚊和白纹伊蚊接种马来丝虫微丝蚴后酚氧化酶活性的变化(英文)

酚氧化酶在昆虫抵抗外来异物黑化和成囊的免疫反应中起重要作用。中华按蚊和白纹伊蚊分别经胸内接种马来丝虫微丝蚴和生理盐水后,测定蚊血淋巴中酚氧化酶活性。结果表明:未接种的两种蚊,其1日龄蚊的酚氧化酶活性显著高于3、12和16日龄蚊。1日龄中华按蚊接种微丝蚴24h后,其酚氧化酶活性比未接种蚊和接种生理盐水蚊高2—3倍。1日龄白纹伊蚊接种微丝蚴24h后,其酚氧化酶活性是未接种蚊的7倍。白纹伊蚊酚氧化酶活性的免疫激活水平显著高于中华按蚊。讨论了中华按蚊和白纹伊蚊酚氧化酶活性的差异与其免疫力差异的关系。

PHENOL OXIDASE ACTIVITY IN ANOPHELES SINENSIS AND AEDES ALBOPICTUS EXPOSED TO MICROFILARIAE OF BRUGIA MALAYI

Phenol oxidase (PO) activity was investigated in cell-free hemolymph collected from Anopheles sinensis and Aedes albopictus female adults intrathoracically inoculated with Brugia malayi microfilariae (mff). inoculated with saline alone, or from uninoculated mosquitoes. The activity of PO from uninoculated 1-day-old mosquitoes was significantly greater than those of mosquitoes on 3. 12 and 16 day. PO activity in mff-inoculated A. sinensis at 24 h postinoculation (PI) was 2-3 times higher as compared with uninoculated or saline-inoculated mosquitoes. Inoculation of B. malayi mff into A. albofictus elicited 7-fold increase in PO activity at 24 h PI as compared with uninoculated mosquitoes. Im-mune-activated levels of PO activity in A. albofnctus was significantly higher as compared with those seen in A. sinensis. The relationship of observed differences in PO activity to differences in immunological capa-bility between A. sinensis and A. albopictus is discussed.