miR-324-5p、miR-605-3p在脑胶质瘤组织的表达及与临床病理参数和预后的关系

摘要 目的：探讨微小核糖核酸（miRNA）-324-5p、miR-605-3p在脑胶质瘤组织的表达及与临床病理参数和预后的关系。方法：选取2018年1月～2019年12月徐州医科大学附属医院收治的90例脑胶质瘤患者。收集术中部分瘤组织和瘤旁组织，采用实时荧光定量聚合酶链式反应（qRT-PCR）检测miR-324-5p、miR-605-3p表达。根据脑胶质瘤组织中miR-324-5p、miR-605-3p表达的平均值分为高表达组和低表达组，采用Kaplan-Meier法分析不同miR-324-5p、miR-605-3p表达脑胶质瘤患者生存情况，采用多因素Cox回归分析脑胶质瘤患者预后的影响因素。结果：与瘤旁组织比较，脑胶质瘤组织中miR-324-5p、miR-605-3p表达降低（P＜0.05）。不同分化程度、淋巴结转移、世界卫生组织（WHO）中枢神经系统肿瘤分类的脑胶质瘤患者miR-324-5p、miR-605-3p表达比较有差异（P＜0.05）。90例脑胶质瘤患者3年总生存率为36.67%（33/90）。Kaplan-Meier生存曲线分析显示，miR-324-5p高表达组、miR-605-3p高表达组总生存率高于miR-324-5p低表达组、miR-605-3p低表达组（P＜0.05）。多因素Cox回归分析显示，低分化、淋巴结转移和WHO中枢神经系统肿瘤分类Ⅲ～Ⅳ级为脑胶质瘤患者死亡的独立危险因素，miR-324-5p和miR-605-3p升高为独立保护因素（P＜0.05）。结论：脑胶质瘤组织中miR-324-5p、miR-605-3p呈低表达，与分化程度、淋巴结转移、WHO中枢神经系统肿瘤分类有关，miR-324-5p、miR-605-3p低表达还可导致不良预后。

Expressions of miR-324-5p and miR-605-3p of Glioma Tissue and Their Relationship with Clinical Pathological Parameters and Prognosis

ABSTRACT Objective: To explore the expressions of microribonucleic acid (miRNA)-324-5p and miR-605-3p of glioma tissue and their relationship with clinical pathological parameters and prognosis. Methods: 90 patients with glioma who were admitted to the Affiliated Hospital of Xuzhou Medical University from January 2018 to December 2019 were selected. Some glioma tissues and adjacent tissues during surgery were collected, and the expressions of miR-324-5p and miR-605-3p were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The patients were divided into high expression group and low expression group according to the average expressions of miR-324-5p and miR-605-3p of glioma tissue, the survival of patients with glioma with different expressions of miR-324-5p and miR-605-3p were analyzed by the Kaplan-Meier method, and the prognostic factors of patients with glioma was analyzed by multivariate Cox regression. Results: Compared with adjacent tissues, the expressions of miR-324-5p and miR-605-3p of glioma tissue decreased (P<0.05). There were differences of the expressions of miR-324-5p and miR-605-3p of patients with glioma with different degrees of differentiation, lymph node metastasis, and the World Health Organization (WHO) classification of central nervous system tumors (P<0.05). The 3-year overall survival rate of 90 patients with glioma was 36.67% (33/90). Kaplan- Meier survival curve analysis showed that the overall survival rates of the miR-324-5p high expression group and the miR-605-3p high expression group were higher than those of the miR-324-5p low expression group and the miR-605-3p low expression group (P<0.05). Multivariate Cox regression analysis showed that low differentiation, lymph node metastasis, and WHO classification of central nervous system tumors of grades III-IV were independent risk factors for death of patients with glioma, and elevated miR-324-5p and miR-605-3p were independent protective factors (P<0.05). Conclusion: Low expressions of miR-324-5p and miR-605-3p of glioma tissue, which are related to the degree of differentiation, lymph node metastasis, and WHO classification of central nervous system tumors, and low expressions of miR-324-5p and miR-605-3p can also lead to poor prognosis.