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TaTIP41 and TaTAP46 positively regulate drought tolerance in wheat by inhibiting PP2A activity |
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| Authors: | Jianhui Ma Yuke Geng Hong Liu Mengqi Zhang Shujuan Liu Chenyang Hao Jian Hou Youfu Zhang Daijing Zhang Weijun Zhang Xueyong Zhang Tian Li |
| Institution: | 1. College of Life Sciences, Henan Normal University, Xinxiang, 453007 China
These authors contributed equally to this work.;2. Key Laboratory of Crop Gene Resources and Germplasm Enhancement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081 China College of Life and Environmental Science, Minzu University of China, Beijing, 100081 China These authors contributed equally to this work.;3. Key Laboratory of Crop Gene Resources and Germplasm Enhancement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081 China;4. College of Life Sciences, Henan Normal University, Xinxiang, 453007 China;5. Crop Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan, 750002 China |
| Abstract: | Drought is a major environmental stress limiting global wheat (Triticum aestivum) production. Exploring drought tolerance genes is important for improving drought adaptation in this crop. Here, we cloned and characterized TaTIP41, a novel drought tolerance gene in wheat. TaTIP41 is a putative conserved component of target of rapamycin (TOR) signaling, and the TaTIP41 homoeologs were expressed in response to drought stress and abscisic acid (ABA). The overexpression of TaTIP41 enhanced drought tolerance and the ABA response, including ABA-induced stomatal closure, while its downregulation using RNA interference (RNAi) had the opposite effect. Furthermore, TaTIP41 physically interacted with TaTAP46, another conserved component of TOR signaling. Like TaTIP41, TaTAP46 positively regulated drought tolerance. Furthermore, TaTIP41 and TaTAP46 interacted with type-2A protein phosphatase (PP2A) catalytic subunits, such as TaPP2A-2, and inhibited their enzymatic activities. Silencing TaPP2A-2 improved drought tolerance in wheat. Together, our findings provide new insights into the roles of TaTIP41 and TaTAP46 in the drought tolerance and ABA response in wheat, and their potential application in improving wheat environmental adaptability. |
| Keywords: | ABA response drought tolerance PP2A TaTIP41 TaTAP46 TOR signaling Triticum aestivum |