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OsWR2 recruits HDA704 to regulate the deacetylation of H4K8ac in the promoter of OsABI5 in response to drought stress |
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| Authors: | Yalu Guo Yiqing Tan Minghao Qu Kai Hong Longjun Zeng Lei Wang Chuxiong Zhuang Qian Qian Jiang Hu Guosheng Xiong |
| Affiliation: | 1. Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120 China Guangdong Laboratory for Lingnan Modern Agriculture, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Sciences, South China Agricultural University, Guangzhou, 510642 China These authors contributed equally to this work.;2. Plant Phenomics Research Center, Academy of Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing, 210095 China These authors contributed equally to this work.;3. College of Animal Science and Technology, Southwest University, Chongqing, 402460 China These authors contributed equally to this work.;4. Plant Phenomics Research Center, Academy of Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing, 210095 China;5. Yichun Academy of Sciences, Yinchun, 336000 China;6. Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050022 China;7. Guangdong Laboratory for Lingnan Modern Agriculture, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Sciences, South China Agricultural University, Guangzhou, 510642 China;8. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310000 China |
| Abstract: | Drought stress is a major environmental factor that limits the growth, development, and yield of rice (Oryza sativa L.). Histone deacetylases (HDACs) are involved in the regulation of drought stress responses. HDA704 is an RPD3/HDA1 class HDAC that mediates the deacetylation of H4K8 (lysine 8 of histone H4) for drought tolerance in rice. In this study, we show that plants overexpressing HDA704 (HDA704-OE) are resistant to drought stress and sensitive to abscisic acid (ABA), whereas HDA704 knockout mutant (hda704) plants displayed decreased drought tolerance and ABA sensitivity. Transcriptome analysis revealed that HDA704 regulates the expression of ABA-related genes in response to drought stress. Moreover, HDA704 was recruited by a drought-resistant transcription factor, WAX SYNTHESIS REGULATORY 2 (OsWR2), and co-regulated the expression of the ABA biosynthesis genes NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3 (NCED3), NCED4, and NCED5 under drought stress. HDA704 also repressed the expression of ABA-INSENSITIVE 5 (OsABI5) and DWARF AND SMALL SEED 1 (OsDSS1) by regulating H4K8ac levels in the promoter regions in response to polyethylene glycol 6000 treatment. In agreement, the loss of OsABI5 function increased resistance to dehydration stress in rice. Our results demonstrate that HDA704 is a positive regulator of the drought stress response and offers avenues for improving drought resistance in rice. |
| Keywords: | drought stress HDA704 histone acetylation OsABI5 OsWR2 rice |