Intraplasmid recombination in Streptomyces lividans 66 |
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| Authors: | Carton W Chen Jane F -Y Tsai and Shuang-en Chuang |
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| Institution: | (1) Institute of Microbiology and Immunology, National Yangming Medical College, Taipei, Taiwan, Republic of China;(2) Panlabs Fermtech Division, Panlabs Taiwan, Ltd., PO Box 26-127, Taipei, Taiwan, Republic of China;(3) Present address: The Rockefeller University, 1230 York Ave., Box 32, New York, USA;(4) Present address: Laboratory of Cellular and Molecular Biology, University of Wisconsin, 53706 Madison, WI, USA |
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| Abstract: | Summary An Escherichia coli-Streptomyces shuttle plasmid pIF132 containing two direct mel repeats was constructed. While pIF132 replicated relatively stably in E. coli (Rec+ or recA), its structure was unstable in S. lividans: recombination between the mel repeats resulted in a smaller plasmid, pIF138. Furthermore, pIF132 formed oligomers extensively in E. coli but not in S. lividans. |
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| Keywords: | Recombination Plasmid Streptomyces Escherichia coli |
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