Microsomal Flavin-Containing Monooxygenase Activity in Rat Corpus Striatum |
| |
Authors: | Michael W. Duffel Stephen G. Gillespie |
| |
Affiliation: | Division of Medicinal Chemistry and Natural Products, College of Pharmacy, University of Iowa, Iowa City, Iowa, U.S.A. |
| |
Abstract: | Microsomal fractions isolated from rat corpus striatum catalyze the oxidation of thiobenzamide to the sulfoxide. The rate of thiobenzamide sulfoxidation is 6.9 +/- 4.8 nmol(min)-1 (mg microsomal protein)-1. The reaction is inhibited by an excess of sulfur- and nitrogen-containing substrates for the microsomal flavin-containing monooxygenase. These inhibitors of thiobenzamide sulfoxidation include methimazole, cysteamine, and trimethylamine. Enzyme activity is also destroyed by treatment of the microsomal preparation at 60 degrees for 1 min. In parallel experiments, rat liver microsomes exhibit similar inhibition characteristics. The data indicate the presence in corpus striatum of a microsomal monooxygenase with catalytic properties of the hepatic microsomal flavin-containing monooxygenase. |
| |
Keywords: | Corpus striatum Microsomal Monooxygenase Sulfoxidation Thiobenzamide N-Oxidation |
本文献已被 PubMed 等数据库收录! |
|