Proteomic profiles of soluble proteins from the esophageal gland in female Meloidogyne incognita |
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Authors: | Xin-Rong Wang Yovany A Moreno Han-Rong Wu Chao Ma Yun-feng Li Jin-ai Zhang Chong Yang Si Sun Wei-jie Ma Timothy G Geary |
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Institution: | 1. Laboratory of Plant Nematology, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China;2. Institute of Parasitology, McGill University, Ste-Anne-de-Bellevue, Quebec, Canada |
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Abstract: | Meloidogyne incognita can infect multiple plant species. Proteins synthesized in the esophageal glands and secreted through the stylet of plant parasitic nematodes play critical roles in the plant-nematode interactions. Female M. incognita live for approximately 15 days, embedded in a host plant, but their esophageal gland proteins have not yet been comprehensively analyzed. In this study, a new bacterium-contamination-resistant method for collecting soluble proteins from esophageal gland cells (SPEGC) of female M. incognita was established. Approximately 5 μg of freeze-dried proteins could be extracted from 150 female M. incognita. Bands of a one-dimensional SDS–polyacrylamide gel were excised after electrophoresis of 20 μg of protein and were analyzed. Two hundred and forty-six proteins from SPEGC of female M. incognita were identified by LC–MS/MS. Gene Ontology analysis suggests that many of the secreted proteins are involved in protein or carbohydrate metabolism and proteolysis. Some of the SPEGC (46.3%) were predicted to be secreted through classical or non-classical secretory pathways. The described method presents a new approach for the identification of proteins stored in SPEGC of an important plant parasitic nematode. This global proteomic profile of SPEGC provides a basis for future studies to elucidate the functions of proteins secreted from female M. incognita on plant responses. |
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